Biomarkers of brain injury
The present invention relates to the field of biomarkers. More specifically, the present invention relates to biomarkers useful in diagnosing brain injuries. Brain injury can include overt or traumatic brain injury, as well as subclinical brain injury (SCI). In one embodiment, a method for diagnosing SCI in a patient comprises (a) collecting a sample from the patient; (b) measuring the levels of a panel of biomarkers in the sample collected from the patient, wherein the panel of biomarkers comprises ASTN1, BAI3, CNDP1, ERMIN, GFAP, GRM3, KLH32, MAGE2, NRG3, NRGN, OMG, SLC39A12, RTN1, and MT3; and (c) comparing the levels of the panel of biomarkers with predefined levels of the same panel of biomarkers that correlate to a patient having SCI and predefined levels of the same panel of biomarkers that correlate to a patient not having SCI, wherein a correlation to one of the predefined levels provides the diagnosis.
1. A method of detecting brain injury in a patient, the method comprising the steps of:
a. contacting a biological sample from the patient with an antibody that specifically binds biomarker glial fibrillary acidic protein (GFAP), an antibody that specifically binds biomarker neurogranin (NRGN) and an antibody that specifically binds one of Astrotactin 1 (ASTN1), Brain Angiogenesis Inhibitor 3 (BAI3), Carnosine Dipeptidase 1 (CNDP1), Ermin (ERMN), Glutamate Receptor Metabotropic 3 (GRM3), Ketch-Like Protein 32 (KLH32), Melanoma Antigen Family E2 (MAGE2), Neuregulin 3 (NRG3), Oligodendrocyte Myelin Glycoprotein (OMG), Solute Carrier Family 39 (zinc transporter) member 12 (SLC39A12), and reticulon 1 (RTN1) in the sample; and
b. detecting binding of the antibodies to the biomarkers, wherein detection of an increase in the levels of said biomarkers relative to an appropriate control detects brain injury in the patient.
2. The method of claim 1 , wherein the biological sample is a blood, plasma, serum, cerebrospinal fluid (CSF), or urine sample.
3. The method of claim 1 , wherein the brain injury is subclinical brain injury or traumatic brain injury.
4. A method of detecting traumatic brain injury in a patient,
the method comprising the steps of:
a. contacting a blood, serum, or plasma sample from the patient with an antibody that specifically binds biomarker GFAP, an antibody that specifically binds biomarker MT3, an antibody that specifically binds biomarker NRGN, and an antibody selected from the group consisting of antibodies that specifically binds a biomarker of brain injury selected from the group consisting of Astrotactin 1 (ASTN1), Brain Angiogenesis Inhibitor 3 (BAI3), Carnosine Dipeptidase 1 (CNDP1), Ermin (ERMN), Glutamate Receptor Metabotropic 3 (GRM3), Ketch-Like Protein 32 (KLH32), Melanoma Antigen Family E2 (MAGE2), Neuregulin 3 (NRG3), Oligodendrocyte Myelin Glycoprotein (OMG), Solute Carrier Family 39 (zinc transporter) member 12 (SLC39A12) and reticulon 1 (RTN1) in the sample; and
b. detecting binding of the antibodies to the biomarkers, wherein detection of said biomarkers relative to an appropriate control detects traumatic brain injury in the patient.