Substance-encapsulating vesicle and process for producing the same
Provided is a method for easily and efficiently producing encapsulated substance vesicles wherein a substance is encapsulated in the cavity of vesicles obtained by polymer self-assembly. Empty vesicles that have membranes comprising a first polymer that is a block copolymer with uncharged hydrophilic segments and a first kind of charged segments and a second polymer with a second kind of charged segments that carry a charge that is the opposite of said first kind of charged segments as well as spaces that are enclosed by said membranes are mixed in an aqueous medium with the substance that is to be encapsulated in the spaces.
1. A vesicle comprising:
a membrane containing a first polymer, which is a block copolymer having an uncharged hydrophilic segment and a first charged segment, and a second polymer, which has a second charged segment charged oppositely to the first charged segment, said membrane defining a cavity surrounded thereby and having a first charged segment to second charged segment ratio of 0.3 to less than 3.00; and
a charged substance encapsulated in the cavity.
2. A vesicle according to claim 1 , wherein the membrane has a trilaminar structure comprising an outer layer, an intermediate layer, and an inner layer.
3. A vesicle according to claim 1 , wherein the charged substance is selected from the group consisting of biomolecules, organic compounds and inorganic substances.
4. A vesicle according to claim 1 , wherein the first polymer is a block copolymer containing an uncharged hydrophilic segment and an anionic segment, and the second polymer is a block copolymer containing an uncharged hydrophilic segment and a cationic segment and/or a polymer containing a cationic segment but no uncharged hydrophilic segment.
5. A vesicle according to claim 1 , wherein the first polymer is a block copolymer containing an uncharged hydrophilic segment and a cationic segment, and the second polymer is a block copolymer containing an uncharged hydrophilic segment and an anionic segment and/or a polymer containing an anionic segment but no uncharged hydrophilic segment.
6. A vesicle according to claim 4 or 5 , wherein the anionic segment is nucleic acid.
7. A vesicle according to claim 4 or 5 , wherein the cationic segment is polyamine.
8. A vesicle according to claim 4 or 5 , wherein the uncharged hydrophilic segment is polyalkylene glycol.