Nanoparticle complexes of anti-CD20 antibodies, albumin and paclitaxel
Described are stable nanoparticle complexes of 100 nm to 900 nm comprising paclitaxel, albumin and a polypeptide which binds CD20 (such as rituximab). The complexes are suitable for the treatment of cancer, including lymphoma.
1. An antibody-albumin nanoparticle complex comprising paclitaxel, albumin and an antibody with specificity for CD20, wherein said nanoparticle complex has been pre-formed in vitro by mixing an aqueous albumin-paclitaxel nanoparticle with the antibody under conditions to form the nanoparticle complex, such that the antibody retains CD20 binding specificity, and wherein the diameter of said nanoparticle complex is between 0.1 micron and 0.9 micron, and further wherein the nanoparticle complex is suitable for treating cancer.
2. The antibody-albumin nanoparticle complex of claim 1 , wherein the antibody is a humanized antibody.
3. A pharmaceutical composition comprising a pharmaceutically acceptable carrier and an effective amount of the antibody-albumin nanoparticle complex of claim 1 , wherein the average diameter of said nanoparticle complexes is between 0.1 micron and 0.9 micron.