Compositions, methods, and kits for eliciting an immune response
The present invention relates to compositions, methods, and kits for eliciting an immune response to at least one CMV antigen expressed by a cancer cell, in particular for treating and preventing cancer. CMV determination methods, compositions, and kits also are provided.
1. A method for eliciting in a subject an immune response to a cancer cell that expresses a cytomegalovirus (CMV) antigen, the method comprising:
selecting a subject in need of treatment related to the presence of the cancer cell; and
administering to the subject a pharmaceutically acceptable composition comprising nucleic acids encoding at least one CMV antigen, wherein the pharmaceutically acceptable composition, when administered to the subject, elicits an immune response to the cell.
2. The method of claim 1 , wherein the at least one CMV antigen is a polypeptide, or an immunogenic fragment thereof, selected from the group consisting of: capsid polypeptides, tegument polypeptides, and envelope polypeptides.
3. The method of claim 1 , wherein the at least one CMV antigen is a polypeptide, or an immunogenic fragment thereof, selected from the group consisting of: phosphoprotein unique long 83 (ppUL83; a/k/a pp65), glycoprotein UL55 (gpUL55; a/k/a gB), UL123 immediate early 1 (IE1) protein, UL122 IE2protein, UL111A (a/k/a mtrII), US28, ppUL32, ppUL65, ppUL80a, ppUL82, ppUL98a, ppUL99, gpUL4 (a/k/a gp48), gpUL16, gpUL18 (a/k/a MHC), gpUL75 (a/k/a gH), gpUL100, gpUL110 (a/k/a gM), gpUL115 (a/k/a gL), pUL46, pUL48, pUL56, pUL86 (a/k/a MCP), glycoprotein unique short 10 (gpUS10), gpUS11, glycoprotein complex II (gcII), gp65, and gp93.
4. The method of claim 1 , wherein the pharmaceutically acceptable composition further comprises at least one adjuvant, or a nucleic acid encoding the adjuvant.
5. The method of claim 4 , wherein the adjuvant is a cytokine selected from the group consisting of: GM-CSF, G-CSF, IL-2, IL-4, IL-7, IL-12, IL-15, IL-21, TNF-α, and M-CSF.
6. The method of claim 4 , wherein the adjuvant comprises incomplete Freund's adjuvant (Montanide ISA 51) or Corynebacterium granulosum P40.
7. The method of claim 1 , wherein the at least one CMV antigen comprises the sequence as set forth in SEQ ID NO:109.
8. The method of claim 1 , wherein the cancer cell is a glioblastoma multiforme (GBM) cancer cell.