Tumor proliferation inhibitor containing ultrasound-sensitive substance and method for inhibiting tumor proliferation by using tumor proliferation inhibitor and low-intensity pulsed ultrasound waves
Provided are a tumor proliferation inhibitor and a method for inhibiting tumor proliferation both of which can be applied to a minimally invasive cancer treatment using low-intensity pulsed ultrasound. The present invention provides a tumor proliferation inhibitor containing an ultrasound-sensitive substance and an acoustic cavitation phenomenon-enhancing substance, and provides a method for inhibiting tumor proliferation that can exhibit a tumor proliferation-inhibitory effect using the tumor proliferation inhibitor in combination with low-intensity pulsed ultrasound of a degree that is used in ultrasound diagnosis, and that can be applied to a minimally invasive cancer treatment using low-intensity pulsed ultrasound.
1. An in vivo method for activating an ultrasound-sensitive substance in tumor tissue for inhibiting the proliferation of a tumor, the method comprising the steps:
administering an acoustic cavitation phenomenon-enhancing substance and a tumor proliferation inhibitor containing an ultrasound-sensitive substance substantially unreactive with light, but reactive with ultrasound waves to a subject with a tumor; and
irradiating the subject to which the ultrasound-sensitive substance has been administered with low-intensity pulsed ultrasound waves which have a power of 0.1 to 10.0 W/cm 2 and 0.1 to 5.0 MHz to activate the ultrasound-sensitive substance,
wherein said acoustic cavitation phenomenon-enhancing substance is an ultrasound contrast agent containing lipid stabilized perfluorobutane microbubbles in an aqueous solution,
wherein the ultrasound-sensitive substance is [7,12-bis(1-(2-(2-hydroxy-ethoxy)ethoxy)ethyl)-3,8,13,17-tetramethylporphyrin-2,18-dipropionato]manganese (DEG) as represented by formula I: