Methods and compositions to enhance activity of Cry endotoxins
Methods and compositions for enhancing the resistance of plants to plant pests are provided. Chimeric pesticidal polypeptides and nucleic acid molecules encoding the chimeric pesticidal polypeptides are provided. The chimeric pesticidal polypeptides comprising a solubility-enhancing polypeptide operably linked to a polypeptide comprising pesticidal activity. The nucleic acid molecules can be used in expression cassettes for making transformed plants with enhanced resistance to plant pests. Further provided are transformed plants, plant tissues, plant cells, other host cells, and seeds as well as pesticidal compositions.
1. A method of enhancing pesticidal activity of a Cry endotoxin, the method comprising operably linking a first amino acid sequence of maltose binding protein to a second amino acid sequence of a Cry endotoxin comprising an amino acid sequence selected from the group consisting of SEQ ID NOS: 8, 10, 12 and 14; whereby a chimeric pesticidal polypeptide is produced; and wherein the chimeric pesticidal polypeptide comprising the first amino acid sequence operably linked to the second amino acid sequence exhibits enhanced pesticidal activity.
2. The method of claim 1 , wherein the maltose binding protein comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 6 and 34.
3. The method of claim 1 or 2, wherein the chimeric pesticidal polypeptide comprises an amino acid sequence selected from the group consisting of the amino acid sequences set forth in SEQ ID NOS: 2, 21, 23, 25, 27 and 33.