Methods and systems for determining a likelihood of adverse prostate cancer pathology
View Patent ↗The present disclosure provides methods that find use in determining a likelihood of adverse prostate cancer pathology in a subject. The methods generally involve detection of one or more diagnostic microRNAs (miRNAs), such as mir-19a, mir-19b, mir-519c-5p, and/or mir-345 in a biological sample from the subject, such as blood or blood product. The detection of one or more such diagnostic miRNAs can be used to determine a likelihood of adverse prostate cancer pathology in a subject. The methods of the present disclosure also find use in facilitating treatment decisions for a subject. Also provided are devices, systems, and kits that may be used in practicing methods of the present disclosure.
1. A method comprising:
determining an amount of each miRNA in a set of miRNAs consisting of:
mir-19a, mir-519c-5p, and mir-345; or
mir-19b, mir-519c-5p, and mir-345,
in a sample of blood or blood product from a male human subject suspected of having prostate cancer, by
a) reverse transcribing RNA from the sample using a primer that hybridizes with mir-19a, mir-19b, mir-519c-5p or mir-345, to generate a reverse transcription product encoding mir-19a, mir-19b, mir-519c-5p or mir-345 and
b) detecting an amount of the reverse transcription product encoding mir-19a, mir-19b, mir-519c-5p or mir-345.
2. The method according to claim 1 , comprising measuring the subject's PSA level.
3. The method according to claim 1 , comprising biopsying the subject's prostate.
4. The method according to claim 1 , wherein the blood product is serum or plasma.
5. The method according to claim 1 , comprising obtaining the sample of blood or blood product from the subject.
6. The method according to claim 1 , wherein the determining comprises performing quantitative real-time PCR to determine the amount of each miRNA in the set of miRNAs.
7. The method according to claim 6 , wherein the quantitative real-time PCR is multiplexed.
8. The method according to claim 1 , comprising administering prostate cancer therapy to the male human subject.
9. The method according to claim 1 , wherein the male human subject is undergoing prostate cancer therapy.
10. The method of claim 1 , wherein the primer is a stem loop primer.
11. The method according to claim 1 , further comprising assaying an amount of one or more reference miRNAs in the sample of blood or blood product.