Method for preparing a vaccine composition comprising at least one antigen and at least one adjuvant
View Patent ↗Method for preparing a vaccine, comprising—a step a) of preparing an adjuvant composition by dispersing, in a physiologically acceptable aqueous solution, at least one inverse latex or a powder of polymer resulting from the atomization of said inverse latex; —a step b) of mixing the composition obtained in step a) into an antigenic medium, intended to form a vaccine composition.
1. A method for preparing a vaccine, said method comprising:
a step a) of preparing an adjuvant composition by dispersing, in a physiologically acceptable aqueous solution, at least one inverse latex or a powder of polymer resulting from the atomization of said inverse latex;
wherein the inverse latex comprises at least one branched or crosslinked, anionic polyelectrolyte comprising a totally or partially salified acrylic acid homopolymer and further comprising a step a) (i) of adding one or more water soluble metal cation organic salts
a step b) of mixing the composition obtained in step a) into an antigenic medium comprising an antigen, to form a composition capable of eliciting an immune response to the antigen upon administration to a subject.
2. The method of claim 1 , wherein during step a) at least one additional immunostimulant substance is added.
3. The method of claim 1 , wherein step b) comprises mixing 10% to 20% of the preparation obtained in step a) with 80% to 90% of the antigenic medium.
4. The method of claim 1 , further comprising, during step b):
a step of adding at least one immunostimulant chosen from saponins, animal and/or plant and/or mineral and/or synthetic oils, surfactants, aluminum hydroxide, lecithins and lecithin derivatives.
5. The method of claim 2 , wherein step b) comprises mixing 10% to 20% of the preparation obtained in step a) with 80% to 90% of the antigenic medium.
6. The method of claim 2 , further comprising, during step b):
a step of adding at least one immunostimulant chosen from saponins, animal and/or plant and/or mineral and/or synthetic oils, surfactants, aluminum hydroxide, lecithins and lecithin derivatives.
7. The method of claim 3 , further comprising, during step b):
a step of adding at least one immunostimulant chosen from saponins, animal and/or plant and/or mineral and/or synthetic oils, surfactants, aluminum hydroxide, lecithins and lecithin derivatives.
8. The method of claim 1 , wherein the one or more water-soluble metal cation organic salts comprises manganese gluconate.
9. The method of claim 1 , wherein the formed composition is a vaccine.
10. The method of claim 1 , wherein the formed composition is a vaccine.
11. The method of claim 8 , wherein the formed composition is a vaccine.