Fusion proteins for the treatment of allergic diseases
The present invention relates to a fusion protein comprising a first peptide and a second peptide linked together with a linker, wherein the first peptide is an allergen and the second peptide is a targeting unit and the targeting unit is a FGL-2 C-terminal peptide according to SEQ ID no 1. Provided herein are also uses of said fusion protein as a vaccine for treating allergy, such as shrimp, peanut or mite allergy, as well as a vaccine composition and methods for producing such fusion proteins.
1. A fusion protein comprising a first peptide and a second peptide optionally linked together with a linker, wherein the first peptide is an allergen and the second peptide consists of an amino acid sequence selected from the group consisting of SEQ ID NO:1 and sequences that are at least 95% identical to SEQ ID NO:1.
2. The fusion protein according to claim 1 , wherein the allergen is shrimp tropomyosin Pan b 1 (SEQ ID no 15) or parts or fragments thereof.
3. The fusion protein according to claim 2 , wherein parts or fragments of shrimp tropomyosin comprises a sequence according to any one of SEQ ID no 4, 5, 6, 7, and 8.
4. The fusion protein according to claim 1 , wherein the allergen is shrimp tropomyosin peptide 5 (P5) (SEQ ID no 8).
5. The fusion protein according to claim 1 , wherein the allergen is shrimp tropomyosin peptide 1 (P1) (SEQ ID no 4).
6. The fusion protein according to claim 1 , wherein the allergen is P5 (SEQ ID no 8) and the linker is RADAAP (SEQ ID no 12).
7. The fusion protein according to claim 1 , wherein the allergen is P1 (SEQ ID no 4) and the linker is RADAAP (SEQ ID no 12).
8. The fusion protein according to claim 1 , wherein the allergen is a peanut allergen according to SEQ ID no 55 or a homologue thereof having least about 95%, 96%, 97%, 98% or 99% identity to SEQ ID no 55.
9. The fusion protein according to claim 1 , wherein the allergen is a mite allergen according to SEQ ID no 56 or a homologue thereof having least about 95%, 96%, 97%, 98% or 99% identity to SEQ ID no 56.
10. The fusion protein according to claim 1 , wherein the allergen is a mite allergen according to SEQ ID no 57 or a homologue thereof having least about 95%, 96%, 97%, 98% or 99% identity to SEQ ID no 57.
11. The fusion protein according to claim 1 , wherein said linker is RADAAP (SEQ ID no 12).
12. A vaccine composition comprising the fusion protein of claim 1 .
13. A method for preparing a fusion protein according of claim 1 , comprising
the steps of:
a) providing an isolated first allergen peptide or the nucleotide sequence thereof;
b) providing an isolated second targeting unit peptide or the nucleotide sequence thereof, wherein the second peptide or the nucleotide sequence thereof is selected from the group consisting of a FGL-2 C-terminal peptide consisting of SEQ ID NO:1, sequences that are at least 95% identical to SEQ ID NO:1, a polynucleotide sequence encoding the peptide consisting of SEQ ID NO:1, and a polynucleotide sequence encoding sequences that are at least 95% identical to SEQ ID NO:1;
c) optionally providing a peptide linker or the nucleotide sequence encoding the peptide linker;
d) fusing said isolated first allergen peptide or the nucleotide sequence thereof of a), with said isolated second targeting unit peptide or the nucleotide sequence thereof of b), optionally spaced apart by the linker of c); and
e) optionally isolating said fusion protein.
14. The method according to claim 13 , wherein said isolated allergen is selected from the group consisting of SEQ ID no 4, SEQ ID no 5, SEQ ID no 6, SEQ ID no 7, SEQ ID no 8, SEQ ID no 55, SEQ ID no 56, and SEQ ID no 57.
15. The method according to claim 13 , wherein the linker is selected from the group consisting of SEQ ID no 12 and SEQ ID no 46.
16. A method for inhibiting, preventing and/or treating allergy, comprising administering an effective amount of a fusion protein of claim 1 to a subject in need thereof.
17. The method of claim 16 , wherein said allergy is selected from the group consisting of a shrimp allergy, a peanut allergy, and a mite allergy.