Receptor gene for peptide cancer antigen-specific T cell
The invention provides the nucleotide sequence and amino acid sequence of the CDR3 domain of the T cell receptor (TCR) gene of a WT1-specific cytotoxic T cell (CTL) against WT1 protein. Also provided are a method for testing for and treating cancer using the nucleotide sequence and amino acid sequence, and a chip, primer set, kit, and device for testing for cancer comprising the nucleotide sequence and amino acid sequence.
1. A synthesized cDNA, comprising:
(1) a first nucleotide sequence encoding a complementarity determining region 3 (CDR3) of a Vβ chain of a T-cell receptor (TCR) of a WTI-specific HLA-A*0201-positive cytotoxic T-cell (CTL), wherein the CDR3 confers diversity and specificity of WT1-antigen recognition of the CTL, or
(2) a second nucleotide sequence that is complementary to the first nucleotide sequence,
wherein the cDNA is obtained by reverse transcription of RNA of an HLA-A*0201-WT1 126-134 tetramer + CD3 + CD8 + cell.
2. The cDNA according to claim 1 , wherein the cDNA consists of:
(1) the first nucleotide sequence, or
(2) the second nucleotide sequence.
3. The cDNA according to claim 1 , wherein the cDNA is labelled.
4. The cDNA according to claim 1 , comprising (1) the first nucleotide sequence.
5. The cDNA according to claim 1 , comprising (2) the second nucleotide sequence.
6. A chip comprising a plurality of different isolated polynucleotides immobilized on the chip, each of the plurality of different isolated polynucleotides immobilized on the chip comprising:
a cDNA sequence encoding an entire amino acid sequence of a complementarity determining region 3 (CDR3) of a Vβ chain of a T-cell receptor (TCR) of a WT1-specific HLA-A*0201-positive cytotoxic T-cell (CTL);
an RNA sequence of a nucleotide that is complementary to the full length of the cDNA sequence; or
a DNA sequence of the nucleotide that is complementary to the full length of the cDNA sequence.
7. The chip according to claim 6 , wherein the isolated polynucleotides further comprise a label.
8. The chip according to claim 1 , wherein the label is selected from the group consisting of a fluorescent label, a radioactive label, an enzyme label, and a chromophore label.
9. The chip according to claim 7 , further comprising a labeled or unlabeled sample placed on the chip.
10. The chip according to claim 6 , further comprising a labeled sample placed on the chip.