Particle sorting apparatus, particle sorting method, and non-transitory computer-readable storage medium storing program
The present disclosure provides a particle sorting apparatus, a particle sorting method, and a non-transitory computer-readable storage medium storing program that enable sorting object particles to be sorted with high precision, even when the sorting object particles are large. In the particle sorting apparatus, a charging unit that applies charges to at least a part of liquid droplets ejected from an orifice to generate a fluid stream and a charging control unit that adjusts a charge application end time in the charging unit according to sizes of particles included in the liquid droplets are provided.
1. A particle sorting apparatus, comprising:
a charging unit configured to apply charges to at least a portion of a plurality of liquid droplets ejected from an orifice;
a charging control unit configured to adjust a charge application end time for the applied charges based on a plurality of sizes of a plurality of particles included in the plurality of liquid droplets; and
a vibration element configured to vibrate a liquid that flows through a flow channel terminated by the orifice,
wherein the vibration of the liquid is based on a driving voltage from a voltage supplying unit, and
wherein the driving voltage is based on an image that comprises a state of the liquid prior to the ejection of a liquid droplet.
2. The particle sorting apparatus according to claim 1 , wherein the charging control unit is further configured to change a start time of a charge application waveform based on the plurality of sizes of the plurality of particles.
3. The particle sorting apparatus according to claim 1 , wherein the charging control unit is further configured to change a charge application duration based on the plurality of sizes of the plurality of particles.
4. The particle sorting apparatus according to claim 1 , further comprising:
a forward-scattered light detecting unit including a light source illuminate the plurality of particles that flows along the flow channel and a detector to detect forward-scattered light generated from the plurality of particles by the illumination,
wherein the charging control unit is further configured to adjust the charge application end time based on a detection result received from the forward-scattered light detecting unit.
5. The particle sorting apparatus according to claim 4 ,
wherein the charging control unit is further configured to delay a charge application waveform based on an intensity of the forward-scattered light detected by the forward-scattered light detecting unit, and
wherein the intensity of the forward-scattered light is equal to or more than a threshold value.
6. The particle sorting apparatus according to claim 4 , further comprising:
a delay amount calculating unit configured to calculate a delay amount of a charge application waveform based on an intensity of the forward-scattered light detected by the forward-scattered light detecting unit,
wherein the charging control unit is further configured to delay the charge application waveform based on the delay amount calculated by the delay amount calculating unit.
7. The particle sorting apparatus according to claim 4 ,
wherein the charging control unit is further configured to lengthen a charge application duration based on an intensity of the forward-scattered light, and
wherein the intensity of the forward-scattered light is equal to or more than a threshold value.
8. The particle sorting apparatus according to claim 4 , further comprising:
an application duration calculating unit configured to calculate a charge application duration based on an intensity of the forward-scattered light detected by the forward-scattered light detecting unit,
wherein the charging unit is further configured to apply the charges for the charge application duration calculated by the application duration calculating unit.
9. The particle sorting apparatus according to claim 1 ,
wherein the orifice is present in an exchangeable microchip and
wherein the charging unit further includes a charging electrode to contact one of a sheath liquid or a sample liquid that flows through the flow channel present in the microchip.
10. The particle sorting apparatus according to claim 1 , wherein the orifice is present in a flow cell.
11. A particle sorting method, comprising:
applying charges to at least a portion of a plurality liquid droplets ejected from an orifice;
adjusting a charge application end time based on a plurality of sizes of a plurality of particles included in the plurality of liquid droplets; and
vibrating a liquid flowing through a flow channel terminated by the orifice,
wherein the vibration of the liquid is based on a driving voltage from a voltage supplying unit, and
wherein the driving voltage is based on an image comprising a state of the liquid prior to the ejecting of a liquid droplet.
12. A non-transitory computer-readable medium having stored thereon computer executable instructions that, when executed by a processor, cause a computer to execute operations, the operations comprising:
applying charges to at least a portion of a plurality liquid droplets ejected from an orifice; and
adjusting a charge application end time based on a plurality of sizes of a plurality of particles detected in the plurality of liquid droplets; and
vibrating a liquid flowing through a flow channel terminated by the orifice,
wherein the vibration of the liquid is based on a driving voltage from a voltage supplying unit, and
wherein the driving voltage is based on an image comprising a state of the liquid prior to the ejecting of a liquid droplet.