Electrosprayer for arthropod tagging
Disclosed is an apparatus and associated method for tagging insects and arthropods. According to an exemplary embodiment of this disclosure, an electrosprayer is provided including a nozzle cartridge, a spray chamber removably attached to the nozzle cartridge and a power supply operatively connected to the nozzle cartridge and a grounding plate within the spray chamber to electrically charge droplets expelled from the nozzle which coat one or more insects contained in the spray chamber.
1. An electrosprayer comprising:
a nozzle cartridge having a bottom end, comprising:
a tagging liquid reservoir provided in the nozzle cartridge,
at least one nozzle operatively associated with the tagging liquid reservoir and inserted through at least one hole in the bottom end of the nozzle cartridge, wherein the at least one nozzle does not protrude below the bottom end of the nozzle cartridge, and
a first electrical connector operatively connected to one of the tagging liquid reservoir and the at least one nozzle;
a spray chamber including a chamber body, a spray chamber bottom plate comprising a grounding plate, and a second electrical connector operatively connected to the grounding plate, the chamber body adapted to form a confinement space for tagging a subject provided therein, and the grounding plate adapted to substantially cover a bottom portion of the spray chamber opposite a top portion of the spray chamber;
a head assembly adapted to be removably attached to the top portion of the spray chamber, wherein the nozzle cartridge is removably inserted into a top section of the head assembly, and the head assembly is adapted to direct the at least one nozzle into the top portion of the spray chamber; and
a power supply operatively connected to provide a voltage and current across the first electrical connector and the second electrical connector, thereby charging droplets of a tagging liquid contained within the tagging liquid reservoir, the droplets expelled from the at least one nozzle and directed to the grounding plate, the droplets coating at least a portion of the subject contained within the spray chamber.
2. The electrosprayer according to claim 1 , wherein the nozzle cartridge has a top end comprising a syringe connector.
3. The electrosprayer according to claim 1 , further comprising:
a laser configured to light an area below the nozzle cartridge within the spray chamber, wherein the spray chamber includes two diametrically opposing openings to allow the laser beam to propagate through the spray chamber.
4. The electrosprayer according to claim 3 , wherein the spray chamber includes a third opening to view the laser light.
5. The electrosprayer according to claim 1 , wherein the power supply is configured to apply ten kilovolts or less of voltage.
6. The electrosprayer according to claim 1 , wherein the tagging liquid provided in the tagging liquid reservoir includes at least one tagging agent.
7. The electrosprayer according to claim 6 , wherein the tagging agent includes one or more of a fluorescent dye, quantum dots, molecular beacons, aptamers, proteins, and aqueously suspended metallic particles.
8. The electrosprayer according to claim 1 , wherein the top section of the head assembly includes a third electrical connector configured to operatively connect to the nozzle cartridge first electrical connector.
9. The electrosprayer according to claim 1 , wherein the at least one hole is counter-bored into the bottom end of the nozzle cartridge.
10. The electrosprayer according to claim 1 , wherein the subject is an insect or arthropod.