Polynucleotides and polypeptides involved in plant fiber development and methods of using same
Isolated polynucleotides are provided. Each of the isolated polynucleotides comprise a nucleic acid sequence encoding a polypeptide having an amino acid sequence at least 80% homologous to SEQ ID NO: 121, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 122, 123, 124, 125, 126, 95 or 96, wherein the polypeptide is capable of regulating cotton fiber development. Also provided are methods of using such polynucleotides for improving fiber quality and/or yield of a fiber producing plant, as well as methods of using such polynucleotides for producing plants having increased biomass/vigor/yield.
1. A nucleic acid construct comprising an isolated polynucleotide comprising a nucleic acid sequence encoding a polypeptide having an amino acid sequence at least 95% sequence identity and different codon usage with respect to the polypeptide set forth by SEQ ID NO: 117 and a heterologous promoter for regulating expression of said nucleic acid sequence in a host cell, wherein the isolated polynucleotide is operably linked to said heterologous promoter, wherein a plant transformed with said construct exhibits an increase in biomass.
2. The nucleic acid construct of claim 1 , wherein said polypeptide is at least 99% identical to the polypeptide set forth by SEQ ID NO: 117.
3. The nucleic acid construct of claim 1 , wherein said polypeptide is set forth by SEQ ID NO: 117.
4. The nucleic acid construct of claim 1 , wherein said polynucleotide is set forth by SEQ ID NO: 12.
5. The nucleic acid construct of claim 1 , wherein said promoter is set forth in SEQ ID NO: 91.
6. The nucleic acid construct of claim 1 , wherein said promoter is a constitutive promoter.
7. The nucleic acid construct of claim 1 , wherein said promoter is selected from the group consisting of an inducible promoter, an endothelial cell promoter, a tissue-specific promoter and a developmental stage-specific promoter.
8. A transgenic plant cell comprising a nucleic acid construct comprising an isolated polynucleotide encoding a polypeptide having an amino acid sequence having at least 95% sequence identity and different codon usage with respect to the polypeptide set forth by SEQ. ID NO: 117, wherein a plant transformed with said construct exhibits an increase in biomass, and wherein the isolated polynucleotide is operably linked to a heterologous promoter for regulating expression of said polynucleotide in a plant cell.
9. The transgenic plant cell of claim 8 , wherein said cell forms part of a plant.
10. The transgenic plant cell of claim 8 , wherein the plant is a monocot plant.
11. The transgenic plant cell of claim 8 , wherein the plant is a dicot plant.
12. A transgenic plant comprising the nucleic acid construct of claim 2 .
13. A method of generating a transgenic plant, comprising introducing into a cell of the plant the nucleic acid construct of claim 1 , thereby generating the transgenic plant.
14. A method of improving biomass, yield and/or growth rate of a plant as compared to a non-transformed plant, the method comprising transforming a plant with a nucleic acid construct which comprises an isolated polynucleotide encoding a polypeptide having, an amino acid sequence at least 95% sequence identity and different codon usage with respect to the polypeptide set forth by SEQ ID NO: 117, and wherein the isolated polynucleotide is operably linked to a heterologous promoter for regulating expression of said polynucleotide in a plant cell, wherein expression of said polynucleotide improves biomass, yield and/or growth rate of the plant as compared to the nontransformed plant.
15. A transgenic plant comprising the nucleic acid construct of claim 3 .
16. A transgenic plant comprising the nucleic acid construct of claim 4 .
17. The method of claim 14 , wherein said polypeptide is set forth by SEQ ID NO: 117.
18. The method of claim 14 , wherein said polynucleotide comprises the nucleic acid sequence set forth by SEQ ID NO 12.