Secreted frizzle-related protein 5 (SFRP5) binding proteins
Methods of treating metabolic diseases and disorders using an antigen binding protein specific for the SFRP5 polypeptide are provided. In various embodiments the metabolic disease or disorder is type 2 diabetes, obesity, dyslipidemia elevated glucose levels, elevated insulin levels and diabetic nephropathy.
1. An antigen binding protein that specifically binds SFRP5 (secreted frizzled-related protein5) and comprises:
i. a CDRH1 of SEQ ID NO:23;
ii. a CDRH2 of SEQ ID NO:24;
iii. a CDRH3 of SEQ ID NO:25;
i. a CDRL1 of SEQ ID NO:26;
ii. a CDRL2 of SEQ ID NO:27; and
iii. a CDRL3 of SEQ ID NO:28.
2. The antigen binding protein according to claim 1 , wherein the antigen binding protein comprises a heavy chain variable region (V H ) and/or a light chain variable region (V L ), wherein
a. the V H has at least 90% sequence identity with the amino acid sequence selected from the group consisting of SEQ ID NO: 19 and 20; and
b. the V L has at least 90% sequence identity with the amino acid sequence selected from the group consisting of SEQ ID NO:21 and 22.
3. The antigen binding protein according to claim 2 , wherein the antigen binding protein comprises a heavy chain variable region (V H ) and a light chain variable region (V L ), wherein
a. the V H comprises the amino acid sequence of SEQ ID NO: 19 and the V L comprises the amino acid sequence of SEQ ID NO:21;
b. the V H comprises the amino acid sequence of SEQ ID NO:20 and the V L comprises the amino acid sequence of SEQ ID NO:21;
c. the V H comprises the amino acid sequence of SEQ ID NO: 19 and the V L comprises the amino acid sequence of SEQ ID NO:22; or
d. the V H comprises the amino acid sequence of SEQ ID NO:20 and the V L comprises the amino acid sequence of SEQ ID NO:22.
4. The antigen binding protein according to claim 2 , wherein the antigen binding protein comprises a heavy chain (HC) and/or a light chain (LC), wherein
a. the HC has at least 90% sequence identity with the amino acid sequence selected from the group consisting of SEQ ID NO:15 and 16; and
b. the LC has at least 90% sequence identity with the amino acid sequence selected from the group consisting of SEQ ID NO:17 and 18.
5. The antigen binding protein according to claim 4 , wherein the antigen binding protein comprises a heavy chain (HC) and a light chain (LC), wherein
a. the HC comprises the amino acid sequence of SEQ ID NO: 15 and the LC comprises the amino acid sequence of SEQ ID NO: 17;
b. the HC comprises the amino acid sequence of SEQ ID NO: 16 and the LC comprises the amino acid sequence of SEQ ID NO: 17;
c. the HC comprises the amino acid sequence of SEQ ID NO: 15 and the LC comprises the amino acid sequence of SEQ ID NO: 18; or
d. the HC comprises the amino acid sequence of SEQ ID NO: 16 and the LC comprises the amino acid sequence of SEQ ID NO: 18.
6. A pharmaceutical composition comprising at least one antigen binding protein according to claim 1 , and a pharmaceutically acceptable diluent, carrier, solubilizer, emulsifier, preservative, or adjuvant.
7. A nucleic acid molecule encoding the antigen binding protein of claim 1 .
8. An isolated host cell comprising the nucleic acid of claim 7 .
9. A vector comprising the nucleic acid of claim 7 .
10. An isolated host cell comprising the vector of claim 9 .
11. A method of making an antigen binding protein comprising culturing a host cell of claim 8 or 10 under conditions suitable for synthesizing the antigen binding protein and purifying the antigen binding protein from the host cell.