Matrix for receiving a tissue sample and use thereof
A custom-made matrix suitable for receiving a tissue sample is described, as well as the use thereof to obtain a multiplex histological preparation. The disclosure also relates to a multiplex biopsy array comprising tissue and/or cell samples arranged in a matrix material and to a method for the preparation of a multiplex biopsy array. Methods for preparing blocks of matrix material to be used in multiplex biopsy arrays are also described, as well as methods for loading biopsy samples in the blocks, and methods for treating and processing the blocks to form biopsy arrays. The biopsy arrays made using the block of matrix material can be used to prepare sections and slides for histological procedures, including quantitative analyzes and parallel processing.
1. A matrix suitable for receiving a tissue sample prior to or after paraffin embedding, said matrix comprising:
a blend of 2-14% w/v animal protein, 2.1-20% w/v one or more lipids selected from the and group consisting of animal fat and vegetable oil, 2-15% w/v glycerin, water, a gelling agent, wherein the gelling agent is ionically gelled, and an inorganic buffer in a concentration in the range of from 10 to 600 mM; and
preformed receptacles in the matrix configured to receive at least one tissue sample.
2. The matrix according to claim 1 , wherein the receptacles are horizontally arranged parallel grooves.
3. The matrix according to claim 1 , in combination with a tissue cassette and sponge.
4. The matrix according to claim 1 , wherein the matrix is configured to receive fresh tissue samples in the preformed receptacles.
5. The matrix of claim 1 , wherein the matrix further comprises an antifoaming agent.
6. The matrix of claim 5 , wherein the blend comprises 1-10% w/v of the antifoaming agent.