AAV capsid proteins for nucleic acid transfer
Recombinant adeno-associated viral (AAV) capsid proteins are provided. Methods for generating the recombinant adeno-associated viral capsid proteins and a library from which the capsids are selected are also provided.
1. A nucleotide sequence encoding a capsid protein, wherein the capsid protein has at least 97% sequence identity to SEQ ID NO:29.
2. The nucleotide sequence of claim 1 , wherein the capsid protein has a sequence selected from the group consisting of SEQ ID NO:29, SEQ ID NO:34, and SEQ ID NO:40.
3. The nucleotide sequence of claim 1 , wherein the nucleotide sequence is SEQ ID NO:2.
4. The nucleotide sequence of claim 1 , wherein the nucleotide sequence is SEQ ID NO:7.
5. The nucleotide sequence of claim 1 , wherein the nucleotide sequence is SEQ ID NO:8.
6. The nucleotide sequence of claim 1 , wherein the nucleotide sequence comprises the sequence of SEQ ID NO:13.
7. A plasmid comprising the nucleotide sequence of claim 1 .
8. A plasmid comprising the nucleotide sequence of claim 2 .
9. A recombinant adeno-associated viral (AAV) vector, comprising the nucleotide sequence according to claim 1 .
10. A recombinant AAV vector, comprising the nucleotide sequence of claim 2 .
11. A nucleotide sequence encoding a capsid protein, wherein the nucleotide sequence is selected from the group consisting of SEQ ID NO:2, SEQ ID NO:7, SEQ ID NO:8 and SEQ ID NO:13.
12. A capsid protein having at least 97% sequence identity to the sequence of SEQ ID NO:29.
13. The capsid protein of claim 12 , wherein the capsid protein is selected from the group consisting of SEQ ID NO:29, SEQ ID NO:34, and SEQ ID NO:40.
14. The capsid protein of claim 12 , wherein the capsid protein comprises the sequence of SEQ ID NO:29.
15. A viral particle comprising the capsid protein of claim 12 .
16. A viral particle comprising the capsid protein of claim 13 .