Methods and compositions for genetically detecting improved milk production traits in cattle
An isolated nucleic acid molecule comprising a polymorphic site selected from the group consisting of positions 164, 269, 284, 407 and 989 of SEQ ID NO: 1, an array or a kit comprising the same. Also provided are a method for detecting single nucleotide polymorphism (SNP) in bovine proteinase inhibitor (PI) gene, a method for haplotyping a bovine cell, a method for progeny testing of cattle based on said haplotyping, a method for selectively breeding of cattle based on haplotyping a parent animal. The present invention further provides a method for testing a dairy cattle for its milk production trait, comprising haplotyping its cells, wherein a cattle having haplotypes 1, 3, 4 or 5 indicates that the cattle has desirable milk production trait. Haplotype 1 indicates that the cattle has the most desirable milk production trait.
1. A method for selectively breeding cattle using a multiple ovulation and embryo transfer (MOET) procedure, the method comprising:
superovulating a female bovine animal;
collecting eggs from said superovulated female bovine animal;
in vitro fertilizing said eggs;
implanting the fertilized eggs into another female bovine animal and allowing for an embryo to develop;
detecting the presence of a proteinase inhibitor (PI) gene haplotype in the developing embryo comprising:
(i) detecting the presence of a wildtype haplotype, wherein a wildtype haplotype comprises the nucleotides G, C, G, G and C at the positions corresponding to positions 164, 269, 284, 407 and 989, respectively, of SEQ ID NO: 1;
(ii) detecting the presence of haplotype 1, wherein haplotype 1 comprises the nucleotides A, C, G, C and T at the positions corresponding to positions 164, 269, 284, 407 and 989, respectively, of SEQ ID NO: 1;
(iii) detecting the presence of haplotype 2, wherein haplotype 2 comprises the nucleotides G, T, T, G and T at the positions corresponding to positions 164, 269, 284, 407 and 989, respectively, of SEQ ID NO: 1;
(iv) detecting the presence of haplotype 3, wherein haplotype 3 comprises the nucleotides G, C, G, G and T at the positions corresponding to positions 164, 269, 284, 407 and 989, respectively, of SEQ ID NO: 1;
(v) detecting the presence of haplotype 4, wherein haplotype 4 comprises the nucleotides G, T, T, G and C at the positions corresponding to positions 164, 269, 284, 407 and 989, respectively, of SEQ ID NO: 1;
(vi) detecting the presence of haplotype 5, wherein haplotype 5 comprises the nucleotides G, C, G, G and C at the positions corresponding to positions 164, 269, 284, 407 and 989, respectively, of SEQ ID NO: 1; or
(vii) detecting the presence of haplotype 6, wherein haplotype 6 comprises the nucleotides A, C, G, C and C at the positions corresponding to positions 164, 269, 284, 407 and 989, respectively, of SEQ ID NO: 1;
and terminating the developing embryo if the developing embryo is not haplotype 1, haplotype 3, haplotype 4 or haplotype 5.
2. The method of claim 1 , further comprising terminating the developing embryo if the developing embryo is not haplotype 1.
3. A method for selectively breeding dairy cattle, the method comprising:
obtaining a genomic DNA sample from a bull;
detecting in said genomic DNA the presence of homozygous haplotype 1 in the PI gene, wherein haplotype 1 comprises the nucleotides A, C, G, C and T at the positions corresponding to positions 164, 269, 284, 407 and 989, respectively, of SEQ ID NO: 1; and
using semen from said bull for fertilizing a female animal.
4. The method according to claim 3 , wherein the female animal is in vitro fertilized with semen from said bull.
5. The method according to claim 3 , wherein a MOET procedure is used to fertilize said female animal.
6. The method according to claim 3 , where said female animal is also homozygous for haplotype 1 in the PI gene.