Enzymes, enzyme components and uses thereof
The invention provides nucleic acid molecules which encodes a novel fatty acid desaturase, KCS, KCR and/or LACS from Thraustochytrium aureum and Sphaeroforma arctica . The invention also provides recombinant expression vectors containing the nucleic acid molecules, host cells into which the expression vectors have been introduced, and methods for large-scale production of long chain polyunsaturated fatty acids (LCPUFAs), e.g., ARA, EPA and DHA and for screening for delta-4 desaturases.
1. A method for the manufacture of polyunsaturated fatty acids comprising:
a) cultivating a host cell comprising a polynucleotide encoding a delta-4 desaturase comprising a nucleic acid sequence selected from the group consisting of:
i) a nucleic acid sequence of SEQ ID NO: 78; and
ii) a nucleic acid sequence encoding a polypeptide having at least 80% sequence identity to SEQ ID NO: 79 and having delta-4 desaturase activity,
under conditions which allow for the production of polyunsaturated fatty acids in said host cell, wherein said polynucleotide further comprises a heterologous expression control sequence operatively linked to said nucleic acid sequence; and
b) obtaining said polyunsaturated fatty acids from said host cell.
2. The method of claim 1 , wherein said poly-unsaturated fatty acid is arachidonic acid (ARA), eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA).
3. A method for the manufacture of an oil, lipid or fatty acid composition comprising the steps of the method of claim 1 and the further step of formulating the polyunsaturated fatty acid as oil, lipid or fatty acid composition.
4. The method of claim 1 , wherein the delta-4 desaturase is heterologous to the host cell.