Polypeptides having beta-mannanase activity and methods of use
The present compositions and methods relate to a beta-mannanase from Bacillus hemicellulosilyticus , polynucleotides encoding the beta-mannanase, and methods of make and/or use thereof. Formulations containing the beta-mannanase are suitable for use in hydrolyzing lignocellulosic biomass substrates, especially those comprising a measurable level of galactoglucomannan (GGM) and/or glucomannan (GM).
1. A recombinant nucleic acid which comprises a nucleic acid encoding a recombinant polypeptide comprising the amino acid sequence of SEQ ID NO:2 or SEQ ID NO:3 and a heterologous nucleic acid, wherein the recombinant polypeptide has beta-mannanase activity, and wherein said nucleic acid encoding the recombinant polypeptide is operably linked to the heterologous nucleic acid.
2. The recombinant nucleic acid of claim 1 , wherein the heterologous nucleic acid encodes a signal peptide sequence selected from any one of SEQ ID NOs:13-41.
3. A host cell comprising the recombinant nucleic acid of claim 1 , wherein the host cell is a microorganism.
4. The host cell of claim 3 wherein the heterologous nucleic acid encodes a signal peptide sequence.
5. The host cell of claim 4 wherein the signal peptide sequence is selected from any one of SEQ ID NOs: 13-41.
6. The host cell of claim 3 wherein the host cell is a bacterial cell or a fungal cell.
7. The host cell of claim 3 wherein the host cell is Trichoderma reesei.
8. The recombinant nucleic acid of claim 1 , wherein the recombinant polypeptide comprises the amino acid sequence of SEQ ID NO: 2.
9. The recombinant nucleic acid of claim 1 , wherein the recombinant polypeptide comprises the amino acid sequence of SEQ ID NO: 3.
10. The host cell of claim 3 , wherein the recombinant polypeptide comprises the amino acid sequence of SEQ ID NO: 2.
11. The host cell of claim 3 , wherein the recombinant polypeptide comprises the amino acid sequence of SEQ ID NO: 3.