Fibrosis biomarker assay
A method of bioassay for the quantification of biomarkers useful in the diagnosis of fibrosis disease and prognosis of its development, including biomarkers indicative of the risk of developing fibrosis after a chronic injury is provided. In particular, according to the present invention, biomarkers relating to degradation fragments of Collagen type I, III, IV, V, and VI, elastin, C-reactive protein, and proteoglycans including Biglycan, Decorin, Versican, and Perlecan are found to be useful.
1. A method of detecting peptide fragments comprising an N- or C-terminal neo-epitope formed by cleavage in vivo of a protein by a proteinase in a patient, said method comprising:
a. obtaining a biofluid sample from a human patient; and
b. detecting whether said peptide fragments comprising said N- or C-terminal neo-epitope are present in the biofluid sample by contacting the biofluid sample with an antibody and detecting binding between the peptide fragments and the antibody;
wherein said antibody is raised against a synthetic peptide corresponding to a C-terminal or N-terminal neo-epitope amino acid sequence formed by cleavage of a protein by a proteinase and has specific binding affinity for said C-terminal or N-terminal neo-epitope amino acid sequence, and
wherein said antibody specifically binds a neo-epitope consisting of an N-terminal amino acid sequence present in the peptide fragments produced by cleavage of collagen type I, said peptide fragments comprising an N-terminal sequence selected from the group consisting of:
Collagen I, alpha1
ISVPGP... SEQ ID NO: 23
VPGPMG... SEQ ID NO: 24
PGPMGP... SEQ ID NO: 25
FQGPPG... SEQ ID NO: 27
KNGDDG... SEQ ID NO: 28
ARGLPG... SEQ ID NO: 29
LDGAKG... SEQ ID NO: 31
LPGERG... SEQ ID NO: 32
ATGAAG... SEQ ID NO: 67
VRGEPG... SEQ ID NO: 33
PGAKGA... SEQ ID NO: 34
GIAGAP... SEQ ID NO: 69
GGPPGP... SEQ ID NO: 35
NSGEPG... SEQ ID NO: 36
VQGPPG... SEQ ID NO: 71
DGVAGP... SEQ ID NO: 37
ERGSPG... SEQ ID NO: 38
RGSPGP... SEQ ID NO: 74
LTGSPG... SEQ ID NO: 39
QDGRPG... SEQ ID NO: 40
ARGQAG... SEQ ID NO: 77
RGVPGP... SEQ ID NO: 41
VGPAGK... SEQ ID NO: 42
KDGEAG... SEQ ID NO: 80
ERGEQG... SEQ ID NO: 43
RGEQGP... SEQ ID NO: 44
QGLPGP... SEQ ID NO: 83
PGERGV... SEQ ID NO: 45
ANGAPG... SEQ ID NO: 46
AGLPGP... SEQ ID NO: 86
ARGAPG... SEQ ID NO: 47
PGDRGE... SEQ ID NO: 48
FAGPPG... SEQ ID NO: 75
AKGDAG... SEQ ID NO: 49
PIGNVG... SEQ ID NO: 50
NVGAPG... SEQ ID NO: 78
AAGRVG... SEQ ID NO: 51
PPGPAG... SEQ ID NO: 52
GEVGPP... SEQ ID NO: 81
GADGPA... SEQ ID NO: 53
GPQGIA... SEQ ID NO: 54
IAGQRG... SEQ ID NO: 84
GQRGVV... SEQ ID NO: 55
QRGVVG... SEQ ID NO: 56
RGVVGL... SEQ ID NO: 87
GLPGQR... SEQ ID NO: 57
PGLPGP... SEQ ID NO: 58
EPGKQG... SEQ ID NO: 90
PMGPPG... SEQ ID NO: 59
MGPPGL... SEQ ID NO: 60
LAGPPG... SEQ ID NO: 89
DKGETG... SEQ ID NO: 61
LQGPPG... SEQ ID NO: 62
PSGASG... SEQ ID NO: 92
SAGAPG... SEQ ID NO: 63
RTGDAG... SEQ ID NO: 64
VGPPGP... SEQ ID NO: 99
FDFSF... SEQ ID NO: 65
DFSF... SEQ ID NO: 66
AGQRGV... SEQ ID NO: 95
LPGPGG... SEQ ID NO: 26
QAGVMG... SEQ ID NO: 76
VVGLPG... SEQ ID NO: 98
SGLDGA... SEQ ID NO: 30
PAGERG... SEQ ID NO: 79
GKQGPS... SEQ ID NO: 93
AKGEAG... SEQ ID NO: 68
ARGERG... SEQ ID NO: 82
ARGPAG... SEQ ID NO: 96
IAGAPG... SEQ ID NO: 70
LTGPIG... SEQ ID NO: 85
ASGPAG... SEQ ID NO: 97
LPGPPG... SEQ ID NO: 72
AGPPGA... SEQ ID NO: 88
GPPGPP... SEQ ID NO: 100
AGPKGS... SEQ ID NO: 73
ATGFPG... SEQ ID NO: 91
GPPGPA... SEQ ID NO: 94.
or wherein said antibody specifically binds a neo-epitope consisting of a C-terminal amino acid sequence present in the peptide fragments produced by cleavage of collagen type I, said peptide fragments comprising an C-terminal sequence selected from the group consisting of:
Collagen I, alpha1
...QLSYGY SEQ ID NO: 101
...EKSTGG SEQ ID NO: 102
...PPGPQG SEQ ID NO: 103
...KGHRGF SEQ ID NO: 104
...PSGPRG SEQ ID NO: 105
...APGPQG SEQ ID NO: 106
...APGPAG SEQ ID NO: 107
...FPGAVG SEQ ID NO: 108
...SEGPQG SEQ ID NO: 109
...GANGAP SEQ ID NO: 110
...ANGAPG SEQ ID NO: 46
...SGPQGP SEQ ID NO: 112
...EPGPVG SEQ ID NO: 113
...EPGPTG SEQ ID NO: 114
...RGFPGA SEQ ID NO: 115
...KGPAGE SEQ ID NO: 116
...RGSPGP SEQ ID NO: 74
...LPGAKG SEQ ID NO: 118
...AVGPAG SEQ ID NO: 122
...PPGARG SEQ ID NO: 120
...PGKAGE SEQ ID NO: 121
...APGPDG SEQ ID NO: 125
...PAGPAG SEQ ID NO: 123
...AGPAGE SEQ ID NO: 124
...KDGVRG SEQ ID NO: 128
...RGERGF SEQ ID NO: 126
...PAGPRG SEQ ID NO: 127
...PGPAGF SEQ ID NO: 131
...PAGPTG SEQ ID NO: 129
...TGARGA SEQ ID NO: 130
...SAGPPG SEQ ID NO: 134
...EPGDAG SEQ ID NO: 132
...PAGPPG SEQ ID NO: 133
...GEVGPP SEQ ID NO: 81
...ATGFPG SEQ ID NO: 91
...NAGPPG SEQ ID NO: 136
...PGPQGI SEQ ID NO: 140
...GEKGSP SEQ ID NO: 138
...GAPGTP SEQ ID NO: 139
...IAGQRG SEQ ID NO: 84
...GPQGIA SEQ ID NO: 54
...PQGIAG SEQ ID NO: 142
...GPSGEP SEQ ID NO: 146
...GQRGVV SEQ ID NO55
...RGERGF SEQ ID NO: 126
...PVGPVG SEQ ID NO: 149
...ERGPPG SEQ ID NO147
...RGPPGP SEQ ID NO: 148
...EQGPSG SEQ ID NO: 152
...PQGPRG SEQ ID 150
...HRGFSG SEQ ID NO: 151
...GPPGPP SEQ ID NO: 100
...PRGPPG SEQ ID NO153
...PPGPRG SEQ ID NO: 154
...PPGPPG SEQ ID NO: 119
...GPPSAG SEQ ID NO156
...PPSAGF SEQ ID NO: 157
...QMGPRG SEQ ID NO: 161
...PPGPAG SEQ ID NO52
...TPGPQG SEQ ID NO: 160
...PGADGQ SEQ ID NO: 164
...PGPPGA SEQ ID 162
...QGIAGQ SEQ ID NO: 63
...PPGPKG SEQ ID NO: 167
...AGSPGF SEQ ID NO165
...LPGPSG SEQ ID NO: 166
...PKGPAG SEQ ID NO: 170
...PGERGA SEQ ID NO168
...PMGPPG SEQ ID NO: 59
...GPAGRP SEQ ID NO: 173
...PPGPIG SEQ ID NO174
...SPGEQG SEQ ID NO: 172
...TGDAGP SEQ ID NO: 175.
or wherein said antibody specifically binds a neo-epitope consisting of an N-terminal amino acid sequence present in the peptide fragments produced by cleavage of collagen type IV, said peptide fragments comprising an N-terminal sequence selected from the group consisting of:
Collagen type IV
IDGYRG... SEQ ID NO: 609
MGPPGT... SEQ ID NO: 610
DGLPGS... SEQ ID NO: 611
PGSKGE... SEQ ID NO: 612
RGFPGP... SEQ ID NO: 613
PGPPGL... SEQ ID NO: 614
GPPGPP... SEQ ID NO: 100
GLPGSM... SEQ ID NO: 615
GLPGQQ... SEQ ID NO: 616
PGIGVQ... SEQ ID NO: 618
GIGPPG... SEQ ID NO: 611
PGLPGI... SEQ ID NO: 504
PGPKGF... SEQ ID NO: 621
SPGIPG... SEQ ID NO: 619
PGMQGE... SEQ ID NO: 620
LGSKGE... SEQ ID NO: 617
KGQPGL... SEQ ID NO: 622
RGPPGP... SEQ ID NO: 148
IRGEPG... SEQ ID NO: 626
FPGPPG... SEQ ID NO: 627
GPLGEK... SEQ ID NO: 625
DGVIGM... SEQ ID NO: 629
PGNPGI... SEQ ID NO: 630
PGLKGD... SEQ ID NO: 624
IKGDKG... SEQ ID NO: 631
PGSPGC... SEQ ID NO: 632
PPSDEI... SEQ ID NO: 623
GPPGVP... SEQ ID NO: 633
DQGDQG... SEQ ID NO: 634
GAPGPQ... SEQ ID NO: 445
KGSIGI... SEQ ID NO: 635
GIPGAP... SEQ ID NO: 372
DRGPQG... SEQ ID NO: 442
ERGSPG... SEQ ID NO: 38
LQGIRG... SEQ ID NO: 628
DGGVPN... SEQ ID NO: 638
SGRDGL... SEQ ID NO: 639
PGPMGP... SEQ ID NO: 25
GPPGLM... SEQ ID NO: 643
DGYRGP... SEQ ID NO: 642
AEGLPG... SEQ ID NO: 641
LPGFAG... SEQ ID NO: 644
GIPGMP... SEQ ID NO: 645
PPGRLG... SEQ ID NO: 636
GPPGEK... SEQ ID NO: 640
EKGQKG... SEQ ID NO: 637
.LPGPDG SEQ ID NO: 2214
.PGIPGT SEQ ID NO: 2227
.ILGHVP SEQ ID NO: 2212
.LRGIPG SEQ ID NO: 760
.PGDIVF SEQ ID NO: 2215
.PGLPGQ SEQ ID NO: 2213
.PGFPGA SEQ ID NO: 2218
.GNKGDP SEQ ID NO: 2216
.SGYPGN SEQ ID NO: 2217
.QQGNRG SEQ ID NO: 2221
.PGPRGK SEQ ID NO: 2219
.VSGPPG SEQ ID NO: 2220
.VGQPGP SEQ ID NO: 2222
.PGPPGP SEQ ID NO: 458
.KRGPPG SEQ ID NO: 2223
.GEPGMQ SEQ ID NO: 2224
.SKGEKG SEQ ID NO: 2226
.LHGFPG SEQ ID NO: 2225
.GEPGPP SEQ ID NO: 675.
wherein said antibody specifically binds a neo-epitope consisting of a C-terminal amino acid sequence present in the peptide fragments produced by cleavage of collagen type IV, said peptide fragments comprising an C-terminal sequence selected from the group consisting of:
Collagen type IV
...RGPPGP SEQ ID NO: 148
...SVDHGF SEQ ID NO: 646
...PSVDHG SEQ ID NO: 647
...VDHGFL SEQ ID NO: 648
...PGQPGY SEQ ID NO: 649
...QPGYTN SEQ ID NO: 650
...PGLPGS SEQ ID NO: 651
...GTPSVD SEQ ID NO: 652
...SVGSPG SEQ ID NO: 653
...LPGSMG SEQ ID NO: 654
...GPPGVP SEQ ID NO: 633
...PGFPGL SEQ ID NO: 655
...PGLPGE SEQ ID NO: 656
...GDPGPP SEQ ID NO: 373
...HQGEMG SEQ ID NO: 657
...GPPGLV SEQ ID NO: 658
...PGIPGP SEQ ID NO: 659
...PGFPGT SEQ ID NO: 660
...PGLPGP SEQ ID NO: 58
...DGIPGP SEQ ID NO: 661
...SGPKGY SEQ ID NO: 662
...GIGPPG SEQ ID NO: 611
...PGPRGE SEQ ID NO: 663
...GQGPPG SEQ ID NO: 664
...PGAIGP SEQ ID NO: 668
...KVDMGS SEQ ID NO: 665
...PGIDGV SEQ ID NO: 666
...GPKGPP SEQ ID NO: 671
...PPGPPG SEQ ID NO: 119
...KGHMGE SEQ ID NO: 667
...GPKGLP SEQ ID NO: 670
...SPGPPG SEQ ID NO: 672
...KGLPGP SEQ ID NO: 669
...GSVGYP SEQ ID NO: 673
...PQGPPG SEQ ID NO: 389
...PPGSPG SEQ ID NO: 674
...GEPGPP SEQ ID NO: 675
...AGNPGP SEQ ID NO: 676
...FPGPQG SEQ ID NO: 679
...PGYTNG SEQ ID NO: 678
...SVDHGF SEQ ID NO: 646
...LSGPPG SEQ ID NO: 680
...PGPQGP SEQ ID NO: 453
...GQGPPG SEQ ID NO: 664
...PGAPGL SEQ ID NO: 467
...GVMGTP SEQ ID NO: 681
...PGIKGS SEQ ID NO: 677
...PGPPGP SEQ ID NO: 458
...GVSGPK SEQ ID NO: 682
HVPGML. SEQ ID NO: 2228
LPVPGQ. SEQ ID NO: 2229
LGPPGL. SEQ ID NO: 2230
GVPGQA. SEQ ID NO: 2231
VPGQAQ. SEQ ID NO: 2232
GPDGFL. SEQ ID NO: 2233
QEGPLG. SEQ ID NO: 2234
LPGEVL. SEQ ID NO: 2235
RGIPGF. SEQ ID NO: 2236
NRGLGF. SEQ ID NO: 2238
IPSDTL. SEQ ID NO: 2239
PAGEKG. SEQ ID NO: 2240
GEKGNK. SEQ ID NO: 2241
PVGPPG. SEQ ID NO: 2242
DIVFRK. SEQ ID NO: 2243
PPGPKG. SEQ ID NO: 167
RGKPGM. SEQ ID NO: 2244
PGTRGL. SEQ ID NO: 2245
GEKGSK. SEQ ID NO: 2246
EKGSKG. SEQ ID NO: 2247
SGQPGL. SEQ ID NO: 2248
AGIPQK. SEQ ID NO: 2237.
or wherein said antibody specifically binds a neo-epitope consisting of an N-terminal amino acid sequence present in the peptide fragments produced by cleavage of biglycan, decorin, lumican, versican, or perlecan said peptide fragments comprising an N-terminal sequence selected from the group consisting of:
Biglycan
SVPKEI... SEQ ID NO: 949
GLKLNY... SEQ ID NO: 950
RISEAK... SEQ ID NO: 951
NSGFEP... SEQ ID NO: 952
LKSVPK... SEQ ID NO: 953
AIELED... SEQ ID NO: 954
LRISEA... SEQ ID NO: 958
QCSDLG... SEQ ID NO: 955
EAKLTG... SEQ ID NO: 956
LLDLQN... SEQ ID NO: 961
LTGIPK... SEQ ID NO: 959
LKAVPK... SEQ ID NO: 960
IELEDL... SEQ ID NO: 957
Decorin
IVIELG... SEQ ID NO: 962
DEASGI... SEQ ID NO: 963
VNNKIS... SEQ ID NO: 964
NGLNQM... SEQ ID NO: 965
LHLDGN... SEQ ID NO: 966
LILVNN... SEQ ID NO: 967
KITEIK... SEQ ID NO: 969
GLPPSL... SEQ ID NO: 970
SNPVQY... SEQ ID NO: 971
SSGIEN... SEQ ID NO: 968
Versican
LLASDA... SEQ ID NO: 972
LATVGE... SEQ ID NO: 973
ETTVLV... SEQ ID NO: 974
ENQDAR... SEQ ID NO: 975
NGFDQC... SEQ ID NO: 976
SLTVVK... SEQ ID NO: 977
Lumican
SLEDLQ... SEQ ID NO: 978
LKEDAV... SEQ ID NO: 979
HLQHNR... SEQ ID NO: 980
LQHNRL... SEQ ID NO: 985
Perlecan
SIEYSP... SEQ ID NO: 981
LVNFTR... SEQ ID NO: 982
VSEAVV... SEQ ID NO: 983
EVSEAV... SEQ ID NO: 984.
or wherein said antibody specifically binds a neo-epitope consisting of a C-terminal amino acid sequence present in the peptide fragments produced by cleavage of biglycan, decorin, lumican, versican, or perlecan, said peptide fragments comprising an C-terminal sequence selected from the group consisting of:
Biglycan
...NNDISE SEQ ID NO: 986
...YWEVQP SEQ ID NO: 987
...EDLLRY SEQ ID NO: 988
...RISEAK SEQ ID NO: 951
...KIQAIE SEQ ID NO: 989
...PETLNE SEQ ID NO: 990
...LRKDDF SEQ ID NO: 991
...LLRYSK SEQ ID NO: 992
...ELRKDD SEQ ID NO: 993
...KDLPET SEQ ID NO: 994
...DLLRYS SEQ ID NO: 995
...AFDGLK SEQ ID NO: 996
...LNELHL SEQ ID NO: 997
Decorin
...GTNPLK SEQ ID NO: 998
...EVPDDR SEQ ID NO: 999
...GAFTPL SEQ ID NO: 1000
...SSGIEN SEQ ID NO: 968
...RVDAAS SEQ ID NO: 1001
...LVKLER SEQ ID 1002
...GMKKLS SEQ ID NO: 1003
...KDGDFK SEQ ID NO: 1004
...HLDGNK SEQ ID NO: 1005
...QPSTFR SEQ ID NO: 1006
...AFQGMK SEQ ID NO: 1007
Versican
...CDVMYG SEQ ID NO: 1008
...NGFDQC SEQ ID NO: 976
...QNGINK SEQ ID NO: 1009
...IGQDYK SEQ ID NO: 1010
Lumican
...QLTHNK SEQ ID NO: 1011
...VSAAFK 1012
...GLKSLE 1013
Perlecan
...EDAGSR SEQ ID NO: 1014
...EFREVS SEQ ID NO: 1015
...VAQQDS SEQ ID NO: 1016
...SAKEFR SEQ ID NO: 1017
...LEPEYR SEQ ID NO: 1018.
or wherein said antibody specifically binds a neo-epitope consisting of an N-terminal amino acid sequence present in the peptide fragments produced by cleavage of C-reactive protein, said peptide fragments comprising an N-terminal sequence selected from the group consisting of:
CRP
AFVFPK SEQ ID NO: 1033
SFGGNF SEQ ID NO: 1305
FGQTDM SEQ ID NO: 1306
VSLKAP SEQ ID NO: 1172
KAFVFP SEQ ID NO: 1167
EVFTKP SEQ ID NO: 1201
TDMSRK SEQ ID NO: 1307
MSRKAF SEQ ID NO: 1308
SRKAFV SEQ ID NO: 1309
VFPKES SEQ ID NO: 1310
FPKESD SEQ ID NO: 1311
KESDTS SEQ ID NO: 1312
LSSTRG SEQ ID NO: 1313
SSTRGY SEQ ID NO: 1314
STRGYS SEQ ID NO: 1080
KRQDNE SEQ ID NO: 1315
WSKDIG SEQ ID NO: 1316
SKDIGY SEQ ID NO: 1317
SIILGQ SEQ ID NO: 1318
YLGGPF SEQ ID NO: 1322
ILGQEQ SEQ ID NO: 1320
IYLGGP SEQ ID NO: 1321
KYEVQG SEQ ID NO: 1294
LGGPFS SEQ ID NO: 1323
ALKYEV SEQ ID NO: 1133
YTELSS SEQ ID NO: 1325
VQGEVF SEQ ID NO: 1324
KPQLWP SEQ ID NO: 1157
QEQDSF SEQ ID NO: 1328
ILIFWS SEQ ID NO: 1326
LVGDIG SEQ ID NO: 1327
QDSFGG SEQ ID NO: 1331
RGYSIF SEQ ID NO: 1329
GAEASI SEQ ID NO: 1330
DTSYVS SEQ ID NO: 1334
TIYLGG SEQ ID NO: 1332
EINTIY SEQ ID NO: 1333
SPDEIN SEQ ID NO: 1337
AEASII SEQ ID NO: 1335
TSWESA SEQ ID NO: 1336
FVLSPD SEQ ID NO: 1340
GGPFSP SEQ ID NO: 1338
YEVQGE SEQ ID NO: 1339
IVEFWV SEQ ID NO: 1343
LKKGYT SEQ ID NO: 1341
IILGQE SEQ ID NO: 1319
EVQGEV SEQ ID NO: 1346
ESDTSY SEQ ID NO: 1344
RKAFVF SEQ ID NO: 1342
SLKAPL SEQ ID NO: 1222
FVFPK SEQ ID NO: 1059
SDTSYV SEQ ID NO: 1347
SYATKR SEQ ID NO: 1349
LKAPLT SEQ ID NO: 1173
IFSYAT SEQ ID NO: 1348
WVDGKP SEQ ID NO: 1352
YATKRQ SEQ ID NO: 1350
EFWVDG SEQ ID NO: 1351
GQEQDS SEQ ID NO: 1355
VDGKPR SEQ ID NO: 1353
LGQEQD SEQ ID NO: 1354
LNWRA SEQ ID NO: 1127
QSLVGD SEQ ID NO: 1356
SPNVLN SEQ ID NO: 1357
GEVFTK SEQ ID NO: 1359
LNWRAL SEQ ID NO: 1128
QGEVFT SEQ ID NO: 1358
VRKSLK SEQ ID NO: 1205
VFTKPQ SEQ ID NO: 1153
IFWSKD SEQ ID NO: 1181
ATKRQD SEQ ID NO: 1362
KKGYTV SEQ ID NO: 1360
FSYATK SEQ ID NO: 1361
NWRAL SEQ ID NO: 1253
FWSKDI SEQ ID NO: 1363
VLNWRA SEQ ID NO: 1249
DFVLSP SEQ ID NO: 1366
NWRALK SEQ ID NO: 1364
GSQSLV SEQ ID NO: 1365
INTIYL SEQ ID NO: 1372
VLSPDE SEQ ID NO: 1367
LKYEVQ SEQ ID NO: 1134
WRALKY SEQ ID NO: 1375
KSLKKG SEQ ID NO: 1371
GNFEGS SEQ ID NO: 1369
TKPQLW SEQ ID NO: 1345
RALKYE SEQ ID NO: 1373
FVFPKE SEQ ID NO: 1060
KGYTVG SEQ ID NO: 1296
GPFSPN SEQ ID NO: 1376
FYTELS SEQ ID NO: 1374
TKRQDN SEQ ID NO: 1368
SLKKGY SEQ ID NO: 1370.
or wherein said antibody specifically binds a neo-epitope consisting of a C-terminal amino acid sequence present in the peptide fragments produced by cleavage of C-reactive protein, said peptide fragments comprising an C-terminal sequence selected from the group consisting of:
CRP
AFVFPK SEQ ID NO: 1033
KPQLWP SEQ ID NO: 1157
PDEINT SEQ ID NO: 1377
KESDTS SEQ ID NO: 1312
DSFGGN SEQ ID NO: 1378
VFTKPQ SEQ ID NO: 1153
LTKPLK SEQ ID NO: 1379
SDTSYV SEQ ID NO: 1347
DTSYVS SEQ ID NO: 1334
KDIGYS SEQ ID NO: 1380
STRGYS SEQ ID NO: 1080
YATKRQ SEQ ID NO: 1350
GAEASI SEQ ID NO: 1330
DGKPRV SEQ ID NO: 1381
VDGKPR SEQ ID NO: 1353
SPNVLN SEQ ID NO: 1357
GPFSPN SEQ ID NO: 1376
NFEGSQ SEQ ID NO: 1382
ALKYEV SEQ ID NO: 1133
YEVQGE SEQ ID NO: 1339
LKYEVQ SEQ ID NO: 1134
KAFVFP SEQ ID NO: 1167
VSLKAP SEQ ID NO: 1172
LKAPLT SEQ ID NO: 1173
LKKGYT SEQ ID NO: 1341
LGQEQD SEQ ID NO: 1354
NVNMWD SEQ ID NO: 1383
PRVRKS SEQ ID NO: 1384
TVGSEI SEQ ID NO: 1385
SRKAFV SEQ ID NO: 1309
GYSFTV SEQ ID NO: 1386
IILGQE SEQ ID NO: 1319
EGSQSL SEQ ID NO: 1387
INTIYL SEQ ID NO: 1372
WSKDIG SEQ ID NO: 1316
EQDSFG SEQ ID NO: 1388
NVLNWR SEQ ID NO: 1389
ASGIVE SEQ ID NO: 1390
NTIYLG SEQ ID NO: 1391
VGAEAS SEQ ID NO: 1392
TDMSRK SEQ ID NO: 1307
FVFPKE SEQ ID NO: 1060
QTDMSR SEQ ID NO: 1394
TSYVSL SEQ ID NO: 1396
MSRKAF SEQ ID NO: 1308
PKESDT SEQ ID NO: 1395
QDNEIL SEQ ID NO: 1398
ESDTSY SEQ ID NO: 1344
DNEILI SEQ ID NO: 1397
TVGAEA SEQ ID NO: 1401
NEILIF SEQ ID NO: 1399
YTVGAE SEQ ID NO: 1400
GNFEGS SEQ ID NO: 1369
DIGNVN SEQ ID NO: 1404
FEGSQS SEQ ID NO: 1403
LNWRAL SEQ ID NO: 1128
NWRALK SEQ ID NO: 1364
LNWRA SEQ ID NO: 1127
EVFTKP SEQ ID NO: 1201
VFTKPQ SEQ ID NO: 1153
KYEVQG SEQ ID NO: 1294
RQDNEI SEQ ID NO: 1405
IFWSKD SEQ ID NO: 1181
KRQDNE SEQ ID NO: 1315
VRKSLK SEQ ID NO: 1205
SYVSLK SEQ ID NO: 1407
FTKPQL SEQ ID NO: 1406
TKPLKA SEQ ID NO: 1408
SIFSYA SEQ ID NO: 1409
SLKAPL SEQ ID NO: 1222
GNVNMW SEQ ID NO: 1410
SQSLVG SEQ ID NO: 1411
GSQSLV SEQ ID NO: 1365
GGNFEG SEQ ID NO: 1413
LVGDIG SEQ ID NO: 1327
FGGNFE SEQ ID NO: 1412
RALKYE SEQ ID NO: 1373
KKGYTV SEQ ID NO: 1360
VQGEVF SEQ ID NO: 1324
GKPRVR SEQ ID NO: 1414
PFSPNV SEQ ID NO: 1402
DMSRKA SEQ ID NO: 1415
EILIFW SEQ ID NO: 1416
QGEVFT SEQ ID NO: 1358
FPKESD SEQ ID NO: 1311
IGYSFT SEQ ID NO: 1417
SPDEIN SEQ ID NO: 1337
APLTKP SEQ ID NO: 1393
FWSKDI SEQ ID NO: 1363.
or wherein said antibody specifically binds a neo-epitope consisting of an N-terminal amino acid sequence present in the peptide fragments produced by cleavage of elastin, said peptide fragments comprising an N-terminal sequence selected from the group consisting of:
Elastin
GVPGAI SEQ ID NO: 1898
AIPGGV SEQ ID NO: 1899
GVPGGV SEQ ID NO: 1900
ALGGGA SEQ ID NO: 1901
LGGGAL SEQ ID NO: 1902
GGALGP SEQ ID NO: 1903
PLKPVP SEQ ID NO: 1904
LKPVPG SEQ ID NO: 1905
GLAGAG SEQ ID NO: 1906
GLGAGL SEQ ID NO: 1907
LGAGLG SEQ ID NO: 1908
AGLGAF SEQ ID NO: 1909
LVPGGV SEQ ID NO: 1910
VADAAA SEQ ID NO: 1911
KAAKAG SEQ ID NO: 1912
PVGYPG SEQ ID NO: 1913
ARFPGV SEQ ID NO: 1914
RFPGVG SEQ ID NO: 1915
VGPFGG SEQ ID NO: 1916
GPQPGV SEQ ID NO: 1917
PQPGVP SEQ ID NO: 1918
TTGKLP SEQ ID NO: 1919
LPYGYG SEQ ID NO: 1920
GYGPGG SEQ ID NO: 1921
FGAGAA SEQ ID NO: 1922
GVLPGV SEQ ID NO: 1923
VLPGVG SEQ ID NO: 1924
TPAAAA SEQ ID NO: 1928
VPGAIP SEQ ID NO: 1926
AIPGIG SEQ ID NO: 1927
VGVPGA SEQ ID NO: 1931
PAAAAA SEQ ID NO: 1929
AAAAAA SEQ ID NO: 1930
GIPVVP SEQ ID NO: 1934
AVGPGV SEQ ID NO: 1932
GVPGVG SEQ ID NO: 1933
ARPGVG SEQ ID NO: 1937
IPGAAV SEQ ID NO: 1935
GAAVPG SEQ ID NO: 1936
SVGGVP SEQ ID NO: 1940
RPGVGV SEQ ID NO: 1938
VGGIPT SEQ ID NO: 1939
VGVAPG SEQ ID NO: 1943
VGGVPG SEQ ID NO: 1941
GVGTPA SEQ ID NO: 1942
GVPVAP SEQ ID NO: 1946
VAPGVG SEQ ID NO: 1944
GVAPGV SEQ ID NO: 1945
LGAGIP SEQ ID NO: 1949
GAGIPG SEQ ID NO: 1947
PGGVAA SEQ ID NO: 1948
PGALAA SEQ ID NO: 1952
AKYGAA SEQ ID NO: 1953
AGIPGL SEQ ID NO: 1925
ALGGVG SEQ ID NO: 1955
LGGVGI SEQ ID NO: 1956
YGAAVP SEQ ID NO: 1954
AGPAAA SEQ ID NO: 1958
GPAAAA SEQ ID NO: 1959
GVGIPG SEQ ID NO: 1957
GLGVPG SEQ ID NO: 1961
LGGIPP SEQ ID NO: 1962
FGLVGA SEQ ID NO: 1960
PLGGVA SEQ ID NO: 1964
LGGVAA SEQ ID NO: 1965
LGGVLG SEQ ID NO: 1963
GVGLPG SEQ ID NO: 1967
VGLPGV SEQ ID NO: 1968
GGVAAR SEQ ID NO: 1966
VPGVPV SEQ ID NO: 1970
VPGVGI SEQ ID NO: 1971
LPGVYP SEQ ID NO: 1969
APGVGV SEQ ID NO: 1973
VPGGVA SEQ ID NO: 1974
VGISPE SEQ ID NO: 1972
VPGGVF SEQ ID NO: 1979
YPTGTG SEQ ID NO: 1977
YPGGVL SEQ ID NO: 1975
LGPGGK SEQ ID NO: 1982
GVFYPG SEQ ID NO: 1980
VPGAGV SEQ ID NO: 1978
LAGAGL SEQ ID NO: 1985
GPGGKP SEQ ID NO: 1983
VFYPGA SEQ ID NO: 1981
LGAFPA SEQ ID NO: 1988
AGAGLG SEQ ID NO: 1986
PGGKPL SEQ ID NO: 1984
LGVSAG SEQ ID NO: 1991
AFPAVT SEQ ID NO: 1989
GAGLGA SEQ ID NO: 1987
FPGVGV SEQ ID NO: 1994
VPGVGL SEQ ID NO: 1992
AVTFPG SEQ ID NO: 1990
PGVPLG SEQ ID NO: 1996
KPGAPG SEQ ID NO: 1995
PGVGLP SEQ ID NO: 1993
YGPGGV SEQ ID NO: 1999
GYPIKA SEQ ID NO: 1997
PKAPGV SEQ ID NO: 1493
GAGVPG SEQ ID NO: 2002
AGYPTGSEQ ID NO: 2000
PKLPGG SEQ ID NO: 1998
IPGIGG SEQ ID NO: 2005
AGVPGV SEQ ID NO: 2003
TGVGPQ SEQ ID NO: 2001
GPGFGP SEQ ID NO: 1976
IGGIAG SEQ ID NO: 2006
GVPGVP SEQ ID NO: 2004
VPGVGV SEQ ID NO: 2008
PGFGPG SEQ ID NO: 1950
GIAGVG SEQ ID NO: 2007
AVPGVV SEQ ID NO: 2011
VPVGVA SEQ ID NO: 2009
PGVVGV SEQ ID NO: 1951
GVGAGG SEQ ID NO: 2014
VPGVVS SEQ ID NO: 2012
VPGAGI SEQ ID NO: 2010
FGLVPG SEQ ID NO: 2017
VGAGGF SEQ ID NO: 2015
YGARPG SEQ ID NO: 2013
PGVGLA SEQ ID NO: 2020
GLVPGV SEQ ID NO: 2018
VGVGGI SEQ ID NO: 2016
LRAAAG SEQ ID NO: 2023
GVGLAP SEQ ID NO: 2021
LVPGVG SEQ ID NO: 2019
GIPGLG SEQ ID NO: 2026
LVGAAG SEQ ID NO: 2024
VGLAPG SEQ ID NO: 2022
VGIPGG SEQ ID NO: 2032
LGVGVG SEQ ID NO: 2027
LVPGGP SEQ ID NO: 2025
GLVGAA SEQ ID NO: 2035
AVPGVL SEQ ID NO: 2030
VGVPGL SEQ ID NO: 2028
GGVLGG SEQ ID NO: 2038
IPGGVV SEQ ID NO: 2033
VLGGLG SEQ ID NO: 2031
GVAARP SEQ ID NO: 2041
VGAAGL SEQ ID NO: 2036
VVGAGP SEQ ID NO: 2034
GVYPGG SEQ ID NO: 2044
GAGQFP SEQ ID NO: 2039
LGGLGV SEQ ID NO: 2037
AAVPGV SEQ ID NO: 2047
VAARPG SEQ ID NO: 2042
AFQFPL SEQ ID NO: 2041
VPGVLG SEQ ID NO: 2050
AAGLGG SEQ ID NO: 2048
RPGFGL SEQ ID NO: 2043
ISPEAQ SEQ ID NO: 2053
GQFPLG SEQ ID NO: 2051
FGPGVV SEQ ID NO: 2046
LGALGG SEQ ID NO: 2056
GVLPGA SEQ ID NO: 2054
FPGALV SEQ ID NO: 2049
GKPLKP SEQ ID NO: 2059
PQAAAA SEQ ID NO: 2077
ALVPGG SEQ ID NO: 2052
AGLGAG SEQ ID NO: 2062
VPGVPG SEQ ID NO: 2057
AAAGLG SEQ ID NO: 2029
VTFPGA SEQ ID NO: 2065
IAGVGT SEQ ID NO: 2060
VGAGVP SEQ ID NO: 2055
GLPGVY SEQ ID NO: 2068
VVGVPG SEQ ID NO: 2063
GGLGAL SEQ ID NO: 2058
GAFAGI SEQ ID NO: 2071
AGIPVV SEQ ID NO: 2066
VGAGPA SEQ ID NO: 2061
YTTGKL SEQ ID NO: 2074
GARPGV SEQ ID NO: 2069
LGVGGL SEQ ID NO: 2064
PGVGVA SEQ ID NO: 2075
VAGVPS SEQ ID NO: 2072
FPLGGV SEQ ID NO: 2067
LAPGVG SEQ ID NO: 2078
VGTPAA SEQ ID NO: 2076
PGFGLS SEQ ID NO: 2070
LPYTTG SEQ ID NO: 2045
GPGVVG SEQ ID NO: 2073.
or wherein said antibody specifically binds a neo-epitope consisting of a C-terminal amino acid sequence present in the peptide fragments produced by cleavage of elastin, said peptide fragments comprising an C-terminal sequence selected from the group consisting of:
Elastin
PGGVPG SEQ ID NO: 2079
PGAGLG SEQ ID NO: 2080
GAGLGA SEQ ID NO: 1987
GALGGG SEQ ID NO: 2081
LGGGAL SEQ ID NO: 1902
GGGALG SEQ ID NO: 2082
GLGAFP SEQ ID NO: 2083
LGAFPA SEQ ID NO: 1988
LGAGLG SEQ ID NO: 1908
VPGGVA SEQ ID NO: 1974
ADAAAA SEQ ID NO: 2084
VPTGAG SEQ ID NO: 2087
RFPGVG SEQ ID NO: 1915
VGVLPG SEQ ID NO: 2086
VPLGYP SEQ ID NO: 2089
PKAPGV SEQ ID NO: 1493
GVGPFG SEQ ID NO: 2088
IPGIGG SEQ ID NO: 2005
LPYGYG SEQ ID NO: 1920
AAAAAK SEQ ID NO: 2090
IGGIAG SEQ ID NO: 2006
GIAGVG SEQ ID NO: 2007
AIPGIG SEQ ID NO: 1927
GVPGVG SEQ ID NO: 1933
AAAAKA SEQ ID NO: 2091
VVGVPG SEQ ID NO: 2063
VVSPEA SEQ ID NO: 2094
GVGVPG SEQ ID NO: 2092
PVVPGA SEQ ID NO: 2093
GIPTYG SEQ ID NO: 2096
VGGIPT SEQ ID NO: 1939
GGIPTY SEQ ID NO: 2095
GVGAGG SEQ ID NO: 2014
GVGVGG SEQ ID NO: 2097
GVGGIP SEQ ID NO: 2098
VAPGVG SEQ ID NO: 1944
GFPGFG SEQ ID NO: 2099
FGVGVG SEQ ID NO: 2100
APGVGL SEQ ID NO: 2104
PGVGIS SEQ ID NO: 2101
SPEAQA SEQ ID NO: 2102
VAPGIG SEQ ID NO: 2107
PGVGVA SEQ ID NO: 2075
GVGVAP SEQ ID NO: 2103
AAGLGA SEQ ID NO: 2109
GIGPGG SEQ ID NO: 2105
APGIGP SEQ ID NO: 2106
AGVPGL SEQ ID NO: 2112
RAAAGL SEQ ID NO: 2108
AAAGLG SEQ ID NO: 2029
GAGPAA SEQ ID NO: 2113
GVPGLG SEQ ID NO: 2110
GVPGFG SEQ ID NO: 2111
GLGGLG SEQ ID NO: 2115
VLGGLG SEQ ID NO: 2031
VGIPGG SEQ ID NO: 2032
PAAAAK SEQ ID NO: 2117
PAAAAA SEQ ID NO: 1929
VPGVGG SEQ ID NO: 2114
AARPGF SEQ ID NO: 2118
GLGVPG SEQ ID NO: 1961
PGVGGL SEQ ID NO: 2116
GPGIPG SEQ ID NO: 2121
RPGFGL SEQ ID NO: 2043
GVAARP SEQ ID NO: 2041
ALGPGG SEQ ID NO: 2124
LSPIFP SEQ ID NO: 2119
AQAAAA SEQ ID NO: 2120
KPVPGG SEQ ID NO: 2127
GPGGVA SEQ ID NO: 2122
LGALGG SEQ ID NO: 2056
VTFPGA SEQ ID NO: 2065
GLGALG SEQ ID NO: 2123
PGAIPG SEQ ID NO: 2146
VPQPGA SEQ ID NO: 2130
GALGPG SEQ ID NO: 2125
TPAAAA SEQ ID NO: 1928
AGVKPK SEQ ID NO: 2133
AFPAVT SEQ ID NO: 1989
AVTFPG SEQ ID NO: 1990
YGYGPG SEQ ID NO: 2136
AAAAYK SEQ ID NO: 2128
AAKAGA SEQ ID NO: 2129
GVGTPA SEQ ID NO: 1942
PGVPTG SEQ ID NO: 2131
GVPTGA SEQ ID NO: 2132
AAAAAA SEQ ID NO: 1930
PIKAPK SEQ ID NO: 2134
KLPGGY SEQ ID NO: 2135
GVPGAG SEQ ID NO: 2140
VGTPAA SEQ ID NO: 2076
VPGVPG SEQ ID NO: 2057
ARPGVG SEQ ID NO: 1937
GIGGIA SEQ ID NO: 2137
GTPAAA SEQ ID NO: 2138
YGVGAG SEQ ID NO: 2144
IPVVPG SEQ ID NO: 2139
VGVPGA SEQ ID NO: 1931
VGAGGF SEQ ID NO: 2015
GAGIPG SEQ ID NO: 1947
PEAAAK SEQ ID NO: 2141
GISPEA SEQ ID NO: 2149
IPTYGV SEQ ID NO: 2145
TYGVGA SEQ ID NO: 2143
VPGAPG SEQ ID NO: 2150
AGGFPG SEQ ID NO: 2147
PTYGVG SEQ ID NO: 2142
APGVGV SEQ ID NO: 1973
VGVAPG SEQ ID NO: 1943
GIPGVA SEQ ID NO: 2148
PGIGPG SEQ ID NO: 2152
PGVGLA SEQ ID NO: 2020
VGLAPG SEQ ID NO: 2022
LGVGVG SEQ ID NO: 2027
GAGVPG SEQ ID NO: 2002
LAPGVG SEQ ID NO: 2078
LGGLGA SEQ ID NO: 2157
PGVGVG SEQ ID NO: 2169
GGVAAA SEQ ID NO: 2151
GPAAAA SEQ ID NO: 1959
GLGVGG SEQ ID NO: 2153
PGLGVG SEQ ID NO: 2154
GVGGLG SEQ ID NO: 2159
GLGVGA SEQ ID NO: 2155
ALAAAK SEQ ID NO: 2156
VAARPG SEQ ID NO: 2042
VGAGPA SEQ ID NO: 2061
AGPAAA SEQ ID NO: 1958
IFPGGA SEQ ID NO: 2163
GGLGVG SEQ ID NO: 2158
LGVGGL SEQ ID NO: 2064
AAKAAK SEQ ID NO: 2166
AGQFPL SEQ ID NO: 2160
GGVAAR SEQ ID NO: 1966
PGGVAA SEQ ID NO: 1948
GFGLSP SEQ ID NO: 2161
PIFPGG SEQ ID NO: 2162
VGVPGL SEQ ID NO: 2028
AAKFGA SEQ ID NO: 2164
AAKYGA SEQ ID NO: 2165
PGVLGG SEQ ID NO: 2085
AGLGAL SEQ ID NO: 2167
RPGVGV SEQ ID NO: 1938
GIPGGV SEQ ID NO: 2168
LKPVPG SEQ ID NO: 1905
GGFPGF SEQ ID NO: 2173
IPPAAA SEQ ID NO: 2170
ALVPGG SEQ ID NO: 2052
VGGVPG SEQ ID NO: 1941
ARPGFG SEQ ID NO: 2171
VLPGAR SEQ ID NO: 2172
GVAPGV SEQ ID NO: 1945
GLSPIF SEQ ID NO: 2174
PTGAGV SEQ ID NO: 2175
VAPVGV SEQ ID NO: 2126
GIPVVP SEQ ID NO: 1934
AGAAGK SEQ ID NO: 2176.
or wherein said antibody specifically binds a neo-epitope consisting of an N-terminal amino acid sequence present in the peptide fragments produced by cleavage of collagen type V, said peptide fragments comprising an N-terminal sequence selected from the group consisting of:
Collagen type V
GDPGPP SEQ ID NO: 373
LRGIPG SEQ ID NO: 760
IGPPGI SEQ ID NO: 761
LQGPPG SEQ ID NO: 62
IGSLGH SEQ ID NO: 762
IRGPPG SEQ ID NO: 763
ANGSPG SEQ ID NO: 764
LIGTPG SEQ ID NO: 765
LPGEPG SEQ ID NO: 766
I P GR P G SEQ ID NO: 767
G P DG P P SEQ ID NO: 768
Q P GPSG SEQ ID NO: 769
L K GNEG SEQ ID NO: 770
ERGH P G SEQ ID NO: 771
GP P GEQ SEQ ID NO: 772
F P G P KG SEQ ID NO: 491
PFRFGG SEQ ID NO: 773
ESQAQA SEQ ID NO: 774
LLGPKG SEQ ID NO: 775
QQGN P G SEQ ID NO: 776
K EG P PG SEQ ID NO: 777
IGLIG P SEQ ID NO: 778
G P PG PP SEQ ID NO: 100
LG PP GE SEQ ID NO: 779
G PP G P K SEQ ID NO: 780
SLGH P G SEQ ID NO: 781
KDGI P G SEQ ID NO: 782
PVGEPG SEQ ID NO: 783
LRGI P G SEQ ID NO: 760
KDGIPG SEQ ID NO: 782
ANGS P G SEQ ID NO: 764
LIGT P G SEQ ID NO: 765
P GE P GP SEQ ID NO: 784
KDGIP SEQ ID NO: 813
VLGPQG SEQ ID NO: 814
DGIPGP SEQ ID NO: 661
QMGPPG SEQ ID NO: 802
LLGAPG SEQ ID NO: 785
GLPGLE SEQ ID NO: 786
ALRGPA SEQ ID NO: 810
LALRGP SEQ ID NO: 788
LTGR P G SEQ ID NO: 789
ETGFQG SEQ ID NO: 808
LRGF P G SEQ ID NO: 790
KTGPIG SEQ ID NO: 791
EAGHPG SEQ ID NO: 811
P GP K GN SEQ ID NO: 527
EQGL P G SEQ ID NO: 793
GSKGPM SEQ ID NO: 809
AIGGP P SEQ ID NO: 794
G P NGDP SEQ ID NO: 795
E K GH P G SEQ ID NO: 812
LLGPRG SEQ ID NO: 797
G P DGPP SEQ ID NO: 768
GPKGSI SEQ ID NO: 816
GPKGDP SEQ ID NO: 799
GD P G PP SEQ ID NO: 373
ELGFQG SEQ ID NO: 817
LQGP P G SEQ ID NO: 62
E K GHIG SEQ ID NO: 801
LRGPAG SEQ ID NO: 818
SRGERG SEQ ID NO: 803
EKG K SG SEQ ID NO: 804
EDGERG SEQ ID NO: 815
L P GE P G SEQ ID NO: 766
PQGAIG SEQ ID NO: 806
GIPGEK SEQ ID NO: 787
PP GR P G SEQ ID NO: 792
P G PP GE SEQ ID NO: 796
LLGP K G SEQ ID NO: 775
G PP GEK SEQ ID NO: 640
ERGPNG SEQ ID NO: 798
GPMGLT SEQ ID NO: 807
GPIGE K SEQ ID NO: 805
P GI P GE SEQ ID NO: 800
QMGP P G SEQ ID NO: 802.
or wherein said antibody specifically binds a neo-epitope consisting of a C-terminal amino acid sequence present in the peptide fragments produced by cleavage of collagen type V, said peptide fragments comprising a C-terminal sequence selected from the group consisting of:
Collagen type V
PIGSLG SEQ ID NO: 819
PVGEPG SEQ ID NO: 783
PGPPGE SEQ ID NO: 796
PPGPTG SEQ ID NO: 820
PKGPPG SEQ ID NO: 821
VAGPLG SEQ ID NO: 822
RPGVTG SEQ ID NO: 823
MMPFQF SEQ ID NO: 824
PGAAGP SEQ ID NO: 825
PVGPAG SEQ ID NO: 826
HEGPTG SEQ ID NO: 827
E P GPRG SEQ ID NO: 516
GP P GL P SEQ ID NO: 828
GQGP P G SEQ ID NO: 664
P P GP P G SEQ ID NO: 119
AGSPGE SEQ ID NO: 829
PVGALG SEQ ID NO: 830
EQGL P G SEQ ID NO: 793
YPG P RG SEQ ID NO: 831
H P GQRG SEQ ID NO: 832
GGGGDA SEQ ID NO: 833
LIGP P G SEQ ID NO: 834
GL P GLK SEQ ID NO: 835
PP GPPG SEQ ID NO: 119
P P GPIG SEQ ID NO: 174
KGL P GL SEQ ID NO: 836
P G P PG P SEQ ID NO: 458
QMGPPG SEQ ID NO: 802
LLGAPG SEQ ID NO: 785
R P GVTG SEQ ID NO: 823
QQARLA SEQ ID NO: 837
PP GH P G SEQ ID NO: 838
PQGPRG SEQ ID NO: 150
PLGPPG SEQ ID NO: 839
GEPGLL SEQ ID NO: 840
EVGPLG SEQ ID NO: 841
IMMPFQ SEQ ID NO: 842
GLPGLK SEQ ID NO: 835
PLGPSG SEQ ID NO: 843
AAGPSG SEQ ID NO: 844
PPGPLG SEQ ID NO: 845
PPGSRG SEQ ID NO: 846
PAGP M G SEQ ID NO: 847
P P GSGG SEQ ID NO: 848
PP G P PG SEQ ID NO: 119
GL P GPV SEQ ID NO: 849
L P GPVG SEQ ID NO: 850
K P GPDG SEQ ID NO: 851
L P GPQG SEQ ID NO: 852
VIQPLP SEQ ID NO: 853
RGPNGP SEQ ID NO: 854
PGPQGS SEQ ID NO: 855
E K GPLG SEQ ID NO: 856
PLGP P G SEQ ID NO: 839
P P GPLG SEQ ID NO: 845
TGPKGE SEQ ID NO: 858
GPKGSI SEQ ID NO: 816
GPPGAA SEQ ID NO: 857
P P GPAG SEQ ID NO: 52
P P GPTG SEQ ID NO: 820
LLGAPG SEQ ID NO: 785
PLGPLG SEQ ID NO: 863
P P GSRG SEQ ID NO: 846
PSGLPG SEQ ID NO: 860
LPG PP G SEQ ID NO: 72
KPGPRG SEQ ID NO: 862
PPG P AG SEQ ID NO: 52
P P GL P G SEQ ID NO: 861
P KGP P G SEQ ID NO: 821
QGL P GL SEQ ID NO: 859
P PGSRG SEQ ID NO: 846.
or wherein said antibody specifically binds a neo-epitope consisting of a N-terminal amino acid sequence present in the peptide fragments produced by cleavage of vimentin, said peptide fragments comprising an N-terminal sequence selected from the group consisting of:
Vimentin
LLQDSV SEQ ID NO: 2182
FADLSE SEQ ID NO: 2183
ISLPLP SEQ ID NO: 2184
or wherein said antibody specifically binds a neo-epitope consisting of a C-terminal amino acid sequence present in the peptide fragments produced by cleavage of vimentin, said peptide fragments comprising a C-terminal sequence selected from the group consisting of:
Vimentin
SVPGVR SEQ ID NO: 2185
SVPGVL SEQ ID NO: 2186.
2. A method as claimed in claim 1 , wherein said antibody specifically binds the peptide fragments of CRP comprising an N-terminal sequence KAFVFP . . . (SEQ ID NO: 1167).
3. A method as claimed in claim 1 , wherein said antibody specifically binds the peptide fragments fragments of Collagen I, alpha 1 comprising the C-terminal amino acid sequence . . . KDGVRG (SEQ ID NO: 128).
4. A method as claimed in claim 1 , wherein said antibody specifically binds the peptide fragments of Collagen type IV comprising the C-terminal amino acid sequence . . . VDHGFL (SEQ ID NO: 648).
5. A method as claimed in claim 1 , wherein said antibody specifically binds the peptide fragments fragments of Collagen type IV comprising the N-terminal amino acid sequence ILGHVP . . . (SEQ ID NO: 2212).
6. A method as claimed in claim 1 , wherein said antibody specifically binds the peptide fragments fragments of Collagen type IV comprising the N-terminal amino acid sequence PGDIVF . . . (SEQ ID NO: 2215).
7. A method as claimed in claim 1 , wherein said antibody specifically binds the peptide fragments fragments of Elastin comprising the C-terminal amino acid sequence . . . GISPEA (SEQ ID NO: 2149).
8. A method as claimed in claim 1 , wherein said antibody specifically binds the peptide fragments fragments of Elastin comprising the C-terminal amino acid sequence . . . GIGPGG (SEQ ID NO: 2105).
9. A method as claimed in claim 1 , wherein said antibody specifically binds the peptide fragments fragments of Vimentin comprising the C-terminal amino acid sequence . . . SVPGVR (SEQ ID NO: 2185).
10. A method as claimed in claim 1 , wherein said antibody specifically binds the peptide fragments fragments of Vimentin comprising the C-terminal amino acid sequence . . . SVPGVL (SEQ ID NO: 2186).