Methods and combinations of signaling markers for assessment of disease states
Methods for preparation of cells for analysis of biomarkers are disclosed. In one aspect, the method for preparation of cells for analysis of biomarkers includes contacting a sample that contains a population of cells with at least one modulating substance at a first temperature, thereby producing a modulated cell population; contacting the modulated cell population with at least one antibody that is directed to a cell surface biomarker at a second temperature that is lower than the first temperature, thereby producing an extracellularly stained cell population; and contacting the extracellularly stained cell population with one or more reagents that fixes and permeabilizes the cells, thereby producing a fixed and permeabilized cell population.
1. A method for preparation of cells for analysis of biomarkers, comprising:
(a) contacting a sample that contains a population of cells with at least one modulating substance at a first temperature, thereby producing a modulated cell population, wherein the first temperature is about 37° C.;
(b) contacting the modulated cell population with at least one antibody that is directed to a cell surface biomarker at a second temperature that is lower than the first temperature, thereby producing an extracellularly stained cell population, wherein the second temperature is about 0° C.;
(c) contacting the extracellularly stained cell population with one or more reagents that fixes and permeabilizes the cells, thereby producing a fixed and permeabilized cell population;
(d) contacting the fixed and permeabilized cell populations with at least one antibody that is directed to an intracellular biomarker, wherein the biomarker comprises a phosphoprotein and is analyzed in a multiparametric, phos-flow cell analysis;
wherein the cell population is fixed at about 37° C.
2. A method according to claim 1 , wherein the at least one antibody in step (b) is tagged with one or more metal atoms.
3. A method according to claim 1 , wherein the at least one antibody in step (d) is tagged with one or more metal atoms.
4. A method according to claim 1 , wherein the at least one modulating substance comprises at least one cytokine.
5. A method according to claim 1 , wherein the at least one modulating substance comprises IL3, IL6, and/or IFNa2.
6. A method according to claim 1 , wherein the multiparametric cell analysis method comprises mass cytometry.
7. A method according to claim 1 , wherein the at least one antibody in step (b) is directed to a biomarker selected from a lineage-determining antigen, an activation- and maturation-associated antigen, and a cytokine receptor.
8. A method according to claim 1 , wherein the at least one antibody in step (b) is directed to a biomarker selected from CD8a-144Nd, CD4-145Nd, CD20-147Sm, CD16-Nd148, CD45-154Sm, CD11c-159Tb, CD14-160Gd, CD33-166Er, CD24-168Er, CD3-170Er, CD66-171Yb, CD56-175Lu, CD27-152Sm, CD45RA-153Eu, IgD-161Dy, CD38-167Er, HLA-DR-174Yb, CD25-176Yb, IL3R/CD123-151Eu, and IL7R/CD127-158Gd.
9. A method according to claim 1 , wherein the at least one antibody in step (b) is a panel of antibodies directed to CD8a-144Nd, CD4-145Nd, CD20-147Sm, CD16-Nd148, CD45-154Sm, CD11c-159Tb, CD14-160Gd, CD33-166Er, CD24-168Er, CD3-170Er, CD66-171Yb, CD56-175Lu, CD27-152Sm, CD45RA-153Eu, IgD-161Dy, CD38-167Er, HLA-DR-174Yb, CD25-176Yb, IL3R/CD123-151Eu, and IL7R/CD127-158Gd.
10. A method according to claim 8 , wherein the at least one antibody comprises a metal tag.
11. A method according to claim 9 , wherein each antibody in the panel of antibodies comprises a different metal tag.
12. A method according to claim 1 , wherein the at least one antibody in step (d) is directed to a biomarker selected from pp38 MAPK-157Gd, total IKB-163Dy, pSTAT3-164Dy, pSTAT1-169Tm, pSTAT5-172Yb, and pPLCy2-173Yb.
13. A method according to claim 1 , wherein the at least one antibody in step (d) is a panel of antibodies directed to: pp38 MAPK-157Gd, total IKB-163Dy, pSTAT3-164Dy, pSTAT1-169Tm, pSTAT5-172Yb, and pPLCy2-173Yb.
14. A method according to claim 12 , wherein the at least one antibody comprises a metal tag.
15. A method according to claim 13 , wherein each antibody in the panel of antibodies comprises a different metal tag.
16. A method according to claim 1 , wherein a comprehensive biomarker response profile is generated for cell-specific effects caused by the modulating substance.
17. A method according to claim 1 , wherein cells with high biomarker signaling activities are identified to predict the cause of disease relapse, to guide disease therapy, and/or to predict disease outcome.
18. A method according to claim 1 , wherein the sample that contains a population of cells comprises a cell line, fresh or frozen mononuclear cells, a fresh human sample, a pathologic sample, or a tissue sample selected from blood, marrow, fine needle aspirate, and a tissue biopsy sample.
19. A method according to claim 1 , wherein simultaneous assessment of baseline signaling activities and cell identification is performed.
20. A method according to claim 1 , wherein the sample containing a population of cells is not contacted with the modulating substance as in step (a), thereby obtaining a baseline signaling for the sample.