Translucent smokeless tobacco product
The invention provides a smokeless tobacco product comprising a tobacco extract. In some embodiments, the smokeless tobacco product is translucent. The invention further provides methods for making and using the smokeless tobacco product. In certain embodiments, the smokeless tobacco product comprises isomalt, maltitol syrup, and a translucent tobacco extract prepared by ultrafiltration.
1. A method of preparing a translucent or transparent extract for incorporating into a smokeless tobacco product, comprising:
(i) extracting a tobacco material with an aqueous solvent to form an aqueous tobacco extract;
(ii) ultrafiltering the aqueous tobacco extract to remove components having a molecular weight above about 50,000 Da to form a translucent or transparent tobacco extract; and
(iii) heat treating the tobacco extract prior to or after step (ii) by heating the extract in an aqueous solution comprising L-lysine, L-cysteine, asparaginase, or hydrogen peroxide.
2. The method of claim 1 , wherein the ultrafiltering step comprises passing the aqueous tobacco extract through multiple ultrafiltration membranes.
3. The method of claim 1 , wherein the ultrafiltering step comprises passing the aqueous tobacco extract through two or more membranes having different molecular weight cutoff values.
4. The method of claim 3 , wherein the two or more membranes comprise a first membrane having a molecular weight cutoff of less than about 100,000 Da and a second membrane having a molecular weight cutoff of less than about 50,000 Da.
5. The method of claim 3 , wherein the two or more membranes comprise a first membrane having a molecular weight cutoff of less than about 75,000 Da and a second membrane having a molecular weight cutoff of less than about 50,000 Da.
6. The method of claim 3 , wherein the two or more membranes comprise a first membrane having a molecular weight cutoff of less than about 50,000 Da and a second membrane having a molecular weight cutoff of less than about 5,000 Da to less than about 20,000 Da.
7. The method of claim 3 , wherein the two or more filters or membranes comprise a first membrane having a molecular weight cutoff of less than about 50,000 Da and a second membrane having a molecular weight cutoff of less than about 5,000 Da.
8. The method of claim 1 , wherein the aqueous solvent used in step (i) is water.
9. The method of claim 1 , further comprising diluting the aqueous tobacco extract with water prior to the ultrafiltering step.
10. The method of claim 1 , wherein the aqueous solution used in step (iii) further comprises NaOH.
11. The method of claim 10 , wherein the aqueous solution used in step (iii) comprises L-lysine and NaOH.
12. The method of claim 1 , wherein the heat treating step comprises heating the tobacco extract at a temperature of at least about 60° C.
13. The method of claim 1 , further comprising concentrating the translucent or transparent extract.
14. The method of claim 13 , wherein the concentrating comprises removing at least a portion of the water from the translucent or transparent extract to produce a syrup form.
15. The method of claim 13 , wherein the concentrating comprises spray drying or freeze drying the translucent or transparent extract.
16. The method of claim 1 , wherein the translucent or transparent extract comprises sugars, organic acids, salts, and alkaloids.
17. The method of claim 1 , further comprising incorporating the tobacco extract into a smokeless tobacco product.