Linkage modified oligomeric compounds
The present invention provides oligomeric compounds comprising at least one neutral methoxypropyl phosphonate modified internucleoside linkage. Such oligomeric compounds have one or more improved properties such as selectivity, potency, improved toxicity profile and or improved proinflammatory profile. Such oligomeric compounds have enhanced stability to exposure to base during synthesis. Certain such oligomeric compounds are useful for hybridizing to a complementary nucleic acid, including but not limited, to nucleic acids in a cell. In certain embodiments, hybridization results in modulation of the amount activity or expression of the target nucleic acid in a cell.
1. A gapped oligomeric compound comprising a contiguous sequence of from 12 to 24 monomer subunits linked by internucleoside linking groups having a gap region of from 6 to 14 contiguous β-D-2′-deoxyribonucleosides located between a 5′-region and a 3′-region wherein the 5′ and 3′-regions each, independently, have from 2 to 8 contiguous monomer subunits selected from RNA-like modified furanosyl nucleosides that each adopt a 3′-endo conformational geometry independently selected from bicyclic nucleosides comprising a bicyclic furanosyl sugar moiety, modified nucleosides comprising a furanosyl sugar moiety having at least one substituent group and modified nucleosides comprising a sugar surrogate group; and
wherein at least one of the internucleoside linking groups has Formula I:
wherein each X is independently O or S.
2. The gapped oligomeric compound of claim 1 wherein the gap region has 10 contiguous monomer subunits and the 5′ and 3′-regions each, independently, have 2, 3 or 5 contiguous monomer subunits.
3. The gapped oligomeric compound of claim 1 comprising 2 internucleoside linking groups of Formula I.
4. The gapped oligomeric compound of claim 3 wherein internucleoside linking groups of Formula I are contiguous.
5. The gapped oligomeric compound of claim 1 comprising 1 internucleoside linking group of Formula I.
6. The gapped oligomeric compound of claim 1 wherein at least one internucleoside linking group of Formula I is located in the gap region between monomer subunits 1 and 2, 2 and 3, 3 and 4 or 4 and 5 counting from the first monomer subunit at the 5′ end of the gap region.
7. The gapped oligomeric compound of claim 1 having two internucleoside linking groups of Formula I located in the gap region between monomer subunits 1 and 3, 2 and 4 or 3 and 5 counting from the first monomer subunit at the 5′ end of the gap region.
8. The gapped oligomeric compound of claim 1 wherein each internucleoside linking group, other than the at least one internucleoside linking group of Formula I is, independently, a phosphodiester or a phosphorothioate internucleoside linking group.
9. The gapped oligomeric compound of claim 1 wherein each internucleoside linking group, other than the at least one internucleoside linking group of Formula I is, a phosphorothioate internucleoside linking group.
10. The gapped oligomeric compound of claim 1 wherein each monomer subunit comprises an optionally protected heterocyclic base moiety independently selected from thymine, cytosine, 5-methylcytosine, adenine and guanine.
11. The gapped oligomeric compound of claim 1 wherein each X is O.
12. The gapped oligomeric compound of claim 1 wherein each X is S.
13. The gapped oligomeric compound of claim 1 wherein the chirality of each internucleoside linking group having Formula I is either R P or S P .
14. The gapped oligomeric compound of claim 1 wherein each modified nucleoside in the 5′ and 3′-regions is, independently, selected from a bicyclic nucleoside comprising a bicyclic furanosyl sugar moiety having a 4′-CH 2 —O-2′ or 4′-CH[(S)—(CH 3 )]—O-2′ bridging group and a modified nucleoside comprising a ribofuranosyl sugar moiety having a 2′—O(CH 2 ) 2 —OCH 3 substituent group.
15. The gapped oligomeric compound of claim 1 wherein the modified nucleosides in the 5′ and 3′-regions comprise at least 2 different types of sugar moieties.
16. The gapped oligomeric compound of claim 1 further comprising one 5′ or 3′-conjugate group comprising a cell targeting moiety and a conjugate linker wherein the conjugate linker has the formula:
—C(═O)—(CH 2 ) 3 —C(═O)N(H)—(CH 2 ) 6 —O—.
17. The gapped oligomeric compound of claim 16 wherein the cell targeting moiety and the conjugate linker have the formula:
18. The gapped oligomeric compound of claim 16 wherein the conjugate group further includes a cleavable moiety between the conjugate linker and the oligomeric compound having the formula:
wherein X is O or S.
19. The gapped oligomeric compound of claim 18 wherein X is O.
20. The gapped oligomeric compound of claim 18 wherein X is S.
21. A method of inhibiting gene expression comprising contacting one or more cells, a tissue or an animal with the gapped oligomeric compound of claim 1 wherein said oligomeric compound is complementary to a target RNA.