Method for producing human monoclonal immunoglobuling antibodies
The present invention a method for producing a human immunoglobulin G (IgG) antibody using a prime-boost regime in a Bone Marrow Liver Thymic (BLT) mouse.
1. A method for producing a human immunoglobulin G (IgG) antibody comprising
(a) subcutaneously administering a prime dose of a peptide selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3 and SEQ ID NO:4 mixed with alum or complete Freund's adjuvant to a Bone Marrow Liver Thymic (BLT) mouse;
(b) subcutaneously administering a first boost of the peptide at least three weeks after the immunization of (a);
(c) subcutaneously administering a second boost of the peptide at least three weeks after the first boost of (b) so that a human IgG is produced to the peptide.
2. The method of claim 1 , further comprising mixing spleen cells of the immunized and boosted BLT mouse with an immortal human cell line to produce a hybridoma.
3. The method of claim 1 , further comprising the step of sacrificing the BLT mouse four to seven days after the second boost.