Methods for obtaining information from single cells within populations using DNA origami nanostructures without the need for single cell sorting
Methods for construction of DNA origami nanostructures, as well as for binding, isolation, linking, and deep sequencing information, such as both of TCR alpha and beta CDR3 mRNA, from individual cells within a mixed population of cells without the need for single cell sorting.
1. A method for sequencing genetic information from individual cells within a mixed population of cells without single-cell sorting, comprising the steps of:
(a) transfecting a cell with a DNA origami nanostructure;
(b) isolating said DNA origami nanostructure from said transfected cell, wherein the DNA origami nanostructure is bound to complementary RNA from said cell;
(c) reverse transcribing said RNA into complementary DNA (cDNA); and
(d) sequencing said complementary DNA.
2. A method for obtaining both TCR alpha and beta CDR3 mRNA sequences from individual cells within a mixed population of cells without single-cell sorting, comprising the steps of:
(a) transfecting a primary T cell with a DNA origami nanostructure having a sequence complementary to TCR alpha and beta constant region mRNA;
(b) isolating said DNA origami nanostructure from said transfected cell, wherein the DNA origami nanostructure is bound to said sequences of complementary mRNA from said cell;
(c) reverse transcribing said mRNA into complementary DNA (cDNA); and
(d) sequencing said complementary DNA.
3. The method of claim 2 , wherein said DNA origami nanostructures are composed of ssDNA M13 phage refolded with complementary ssDNA staple sequences into predetermined shapes with selected staples extended with complementary sequences to TCR alpha and beta constant region mRNA.
4. The method of claim 1 , wherein said step of transfecting comprises electroporation.
5. A method for sequencing two or more nucleic acid sequences from individual cells within a mixed population of cells without single-cell sorting, comprising the steps of:
(a) transfecting a cell with a DNA origami nanostructure;
(b) isolating said DNA origami nanostructure from said transfected cell, wherein the DNA origami nanostructure is bound to two or more sequences of complementary nucleic acids from said cell; and
(c) sequencing said two or more sequences of complementary nucleic acids.
6. The method of claim 5 , further comprising linking said two or more sequences of complementary nucleic acids into a single DNA molecule and sequencing said DNA.
7. The method of claim 5 , wherein said step of transfecting comprises electroporation.