Patent
A novel the Ensifer adhaerens strain isolated from soil and a method of producing psicose using the same are provided.
1. A method of producing psicose from fructose by using an Ensifer sp. strain, comprising:
reacting an Ensifer sp. strain with fructose,
wherein reacting the Ensifer sp. strain with fructose is performed by culturing the Ensifer sp. strain on culture medium containing fructose;
mixing the fructose with at least one selected from the group consisting of: an Ensifer sp. strain cell, an Ensifer sp. strain cell culture, and an Ensifer sp. strain cell lysate; or
contacting the fructose with a support immobilized with at least one selected from the group consisting of an Ensifer sp. strain cell, an Ensifer sp. strain cell culture, and an Ensifer sp. strain cell lysate, and wherein
the Ensifer sp. strain is Ensifer adhaerens.
2. The method of claim 1 , wherein the method further comprises a step of adding at least one metal ion selected from the group consisting of Cu, Mn, Ca, Mg, Zn, Ni, Co, Fe and Al ions.
3. The method of claim 1 , wherein the method is performed without using a buffer solution.
4. The method of claim 1 , wherein the method is performed at pH of 6 to 9 and at a temperature of 40 to 80° C.
5. A composition for producing psicose from fructose, comprising an Ensifer sp. strain,
wherein the Ensifer sp. strain is Ensifer adhaerens , and
wherein the Ensifer adhaerens is Ensifer adhaerens SYG29 deposited as accession number of KCCM11405P.
6. The composition of claim 5 , wherein the Ensifer sp. strain is at least one selected from the group consisting of an Ensifer sp. strain cell, an Ensifer sp. strain cell culture, and an Ensifer sp. strain cell lysate.
7. A Ensifer adhaerens strain with an activity of converting fructose to psicose,
wherein the Ensifer adhaerens is Ensifer adhaerens SYG29 deposited as accession number of KCCM11405P.