Compositions comprising cocktails of antibacterial phages and uses thereof for the treatment of bacterial infections
The present invention is directed to the field of phage therapy for the treatment and control of bacterial infections, in particular diabetic foot infections. More specifically, the present invention is directed to novel cocktails of bacteriophage strains F44/10, F1 25/10, F770/05, F510/08, F1 245/05, and/or variants thereof; and methods of using same in the treatment and prevention of bacterial infections, including cutaneous ulcers associated with diabetic foot infections, caused by, e.g., Staphylococcus aureus, Pseudomonas aeruginosa , and/or Acinetobacter baumannii . The cocktails are used as pharmaceutical compositions either alone or in further combination with other therapies, e.g., antibiotics, growth factors, or other standard, as well as non-standard, therapies for diabetic foot infections.
1. A method of treating or reducing the incidence of a bacterial infection in a subject in need thereof comprising administering to said subject a therapeutically effective amount of a pharmaceutical composition, said pharmaceutical composition comprising a pharmaceutically acceptable carrier;
a first and a second purified strain of bacteriophage, each of said strains having a genome which comprises at least 99% sequence identity to the nucleotide sequence selected from the group of consisting of SEQ ID NO:1 and SEQ ID NO:2, and showing antibacterial activity against Staphylococcus aureus;
a third purified strain of bacteriophage having a genome which comprises at least 99% sequence identity to the nucleotide sequence SEQ ID NO:3 and showing antibacterial activity against Pseudomonas aeruginosa;
a fourth purified strain of bacteriophage having a genome which comprises at least 99% sequence identity to the nucleotide sequence SEQ ID NO:4, and showing antibacterial activity against Pseudomonas aeruginosa ; and
a fifth purified strain of bacteriophage having a genome which comprises at least 99% sequence identity to the nucleotide sequence SEQ ID NO:5 and showing antibacterial activity against Acinetobacter baumannii;
wherein each of said first, second, fourth, and fifth bacteriophage strains is present in the composition in an amount about 10 times that of said third bacteriophage strain; and
wherein said bacterial infection is a Staphylococcus aureus infection.
2. The method of claim 1 wherein said bacterial infection is diabetic foot infection.
3. The method according to claim 2 , wherein said treatment comprises topically administering said composition to a cutaneous ulcer associated with said diabetic foot infection, and/or
wherein said administration follows mechanical debridement of said ulcer; and/or
wherein said administration comprises use of at least one of a dressing, an instillation device, and a negative pressure wound therapy device; and/or
wherein said pharmaceutical composition is administered every 4 hours or every 6 hours for an initial 24 hours, and following said initial 24 hours, said pharmaceutical composition is administered every 12 hours or every 24 hours for at least 3 or 4 additional days; and/or
wherein said method is used in combination with a standard therapy for diabetic foot infection, said standard therapy selected from the group consisting of extracellular matrix replacement therapy, moist wound therapy, negative pressure wound therapy, arterial re-vascularization therapy, hyperbaric oxygen therapy, administration of an antibiotic agent, and administration of a growth factor; or
wherein said method is used in combination with a non-standard therapy for diabetic foot infection, wherein said diabetic foot infection is refractory to a standard therapy.
4. The method according to claim 1 , wherein said subject is a human.
5. The method according to claim 3 , wherein said extracellular matrix replacement therapy comprises use of bio-engineered tissue;
wherein said antibiotic agent has antibacterial activity against at least one of Acinetobacter baumannii, Pseudomonas aeruginosa , and Staphylococcus aureus;
wherein said antibiotic agent is administered via systemic administration;
wherein said growth factor is at least one selected from the groups consisting of platelet-derived growth factor, granulocyte colony-stimulating factor, epidermal growth factor, fibroblast growth factor, nerve growth factor, and vascular endothelial growth factor; and/or
wherein said growth factor is administered topically.
6. The method of claim 1 , wherein said composition is formulated for topical application to an area of non-intact skin.
7. The method of claim 6 , wherein said area of non-intact skin is selected from a diabetic ulcer, a cutaneous ulcer, a chronic ulcer, a burn wound, a cellulitis sore, an erysipelas lesion, a decubitus ulcer, and a pressure sore.
8. The method of claim 7 , wherein administration of said composition provides said third phage strain in an amount of about 10 7 phage particles/cm 2 of said area.
9. The method of claim 7 , wherein administration of said composition provides said third phage strain in an amount of about 10 8 phage particles/cm 2 of said area.
10. The method of claim 7 , wherein administration of said composition provides said third phage strain in an amount of about 10 9 phage particles/cm 2 of said area.
11. The method of claim 1 , wherein said bacterial infection is an infection further caused by Pseudomonas aeruginosa.
12. The method of claim 1 , wherein said bacterial infection is an infection further caused by Acinetobacter baumannii.
13. The method of claim 1 , wherein said bacterial infection is an infection further caused by Pseudomonas aeruginosa and Acinetobacter baumannii.