Method of screening gene for 1,4-BDO production
Provided is a screening method of discovering genes capable of increasing 1,4-BDO production on the basis of proteomics data. Over-expression of proteins screened by the method, NCgl0630 (citrate synthase) and NCgl2145 (hypothetical protein), increase 1,4-BDO productivity. The method may lead to screening of a protein associated with 1,4-BDO productivity, thereby increasing 1,4-BDO productivity, and thus, the method may be recognized as being industrially applicable.
1. A Corynebacterium glutamicum microorganism capable of producing 1,4-butanediol (1,4-BDO),
wherein said Corynebacterium glutamicum microorganism is transformed with a nucleic acid comprising a nucleotide sequence encoding an amino acid sequence having at least 95% sequence identity with the amino acid sequence of SEQ ID NO: 3, and exhibits increased expression of the nucleic acid compared to a parent Corynebacterium glutamicum microorganism that is not transformed with the nucleic acid;
wherein the increased expression of the nucleic acid in said Corynebacterium glutamicum microorganism results in increased 1,4-BDO production compared to a parent Corynebacterium glutamicum microorganism capable of 1,4-BDO production that is not transformed with the nucleic acid; and
wherein said Corynebacterium glutamicum microorganism comprises genes encoding coenzyme A-dependent succinate semialdehyde dehydrogenase, 4-hydroxybutyrate dehydrogenase, 4-hydroxybutyryl CoA:acetyl-CoA transferase, and alcohol dehydrogenase.
2. The microorganism of claim 1 , wherein the nucleotide sequence encoding an amino acid sequence having at least 95% sequence identity with the amino acid sequence of SEQ ID NO: 3 is the nucleotide sequence of SEQ ID NO: 4.
3. The microorganism of claim 1 , wherein the microorganism further comprises a nucleic acid comprising a nucleotide sequence encoding citrate synthase.
4. A method of producing 1,4-BDO comprising:
culturing the microorganism of claim 1 , in a culture solution to produce 1,4-BDO; and
obtaining the 1,4-BDO from the culture solution.