Split peroxidases and methods of use
An imaging method utilizing a split peroxidase is described herein. Imaging methods involve contacting a cell with a split peroxidase and a substrate thereof to allow conversion of a substrate into a product via an enzymatic reaction catalyzed by the reconstitute split peroxidase. Also disclosed herein are split peroxidases, related products and kits.
1. An isolated component of a split peroxidase, wherein the split peroxidase is two fragments, a first fragment and a second fragment, which together form a whole peroxidase with a cleavage site, comprising the first fragment of the whole peroxidase fused to a protein of interest, optionally through a linker, wherein the cleavage site is a solvent exposed loop region of the whole peroxidase.
2. The isolated component of a split peroxidase of claim 1 , wherein the protein of interest is a mitochondrial protein, mitochondrial matrix protein, a mitochondrial intermembrane space protein, a mitochondrial inner membrane protein, a mitochondrial outer membrane protein (facing cytosol), a Golgi protein, an endoplasmic reticulum lumen protein, an endoplasmic reticulum membrane protein (facing cytosol), a cell surface protein, a secreted protein, a nuclear protein, a vesicle protein, a cell skeleton protein, a cell skeleton-binding protein, a motor protein, a gap junction protein, a chromatin-organizing protein, a transcription factor protein, a DNA polymerase protein, a ribosomal protein, a synaptic protein, or an adhesion protein.
3. The isolated component of a split peroxidase of claim 1 , further comprising a cellular localization signal peptide linked to the split peroxidase or the protein of interest.
4. The isolated component of a split peroxidase of claim 3 , wherein the cellular localization signal peptide is an ER-targeting signal peptide, a Golgi-targeting signal peptide, a mitochondria-targeting signal peptide, a nuclear localization signal peptide, or a nuclear export signal peptide and/or wherein the cellular localization signal peptide comprises an amino acid sequence selected from the group consisting of:
(SEQ ID NO: 4)
DPVVVLGLCLSCLLLLSLWKQSYGGG,
(SEQ ID NO: 5)
MLATRVFSLVGKRAISTSVCVRAH,
(SEQ ID NO: 6)
LQLPPLERLTLD,
and
(SEQ ID NO: 7)
KDEL.
5. The isolated component of a split peroxidase of claim 3 , wherein the peroxidase has an amino acid sequence selected from SEQ ID NO:1, 2, 3, 8, 9, 10, 11, or 12 and wherein the linker is a flexible amino acid linker.
6. An isolated component of a split peroxidase, wherein the split peroxidase is two fragments, a first fragment and a second fragment, which together form a whole peroxidase with a cleavage site, comprising the first fragment of the whole peroxidase fused to a cellular localization signal, wherein the cleavage site is a solvent exposed loop region of the whole peroxidase.
7. The isolated component of a split peroxidase of claim 6 , wherein the cellular localization signal peptide is an ER-targeting signal peptide, a Golgi-targeting signal peptide, a mitochondria-targeting signal peptide, a nuclear localization signal peptide, or a nuclear export signal peptide.
8. The isolated component of a split peroxidase of claim 6 , wherein the cellular localization signal peptide comprises an amino acid sequence selected from the group consisting of:
(SEQ ID NO: 4)
DPVVVLGLCLSCLLLLSLWKQSYGGG,
(SEQ ID NO: 5)
MLATRVFSLVGKRAISTSVCVRAH,
(SEQ ID NO: 6)
LQLPPLERLTLD,
and
(SEQ ID NO: 7)
KDEL.
9. The isolated component of a split peroxidase of claim 1 , wherein the peroxidase has an amino acid sequence fragment selected from SEQ ID NO:1, 2, 3, 8, 9, 10, 11, or 12.
10. The isolated component of a split peroxidase of claim 1 , wherein the split peroxidase is SEQ ID NO: 13 or 15.
11. The isolated component of a split peroxidase of claim 1 , wherein the split peroxidase is selected from the group consisting of amino acids 1-58 of SEQ ID NO: 8, amino acids 1-308 of SEQ ID NO: 8, amino acids 1-213 of SEQ ID NO: 8, amino acids 214-308 of SEQ ID NO: 8, amino acids 1-50 of SEQ ID NO:11, amino acids 51-249 of SEQ ID NO:11, amino acids 1-200 of SEQ ID NO:11, amino acids 201-249 of SEQ ID NO:11, amino acids 1-50 of SEQ ID NO:12, amino acids 51-249 of SEQ ID NO:12, amino acids 1-200 of SEQ ID NO:12, or amino acids 201-249 of SEQ ID NO:12.
12. A kit, comprising:
a set of split peroxidase components, wherein the split peroxidase components are two fragments, a first fragment and a second fragment, which together form a whole peroxidase with a cleavage site and wherein the cleavage site is a solvent exposed loop region of the whole peroxidase, and instructions for delivering the split peroxidase components to a cell to label one or more proteins of the cell.
13. The kit of claim 12 , further comprising a substrate.