NSP4 inhibitors and methods of use
The invention provides NSP4 inhibitors (such as anti-NSP4 antibodies) and methods of using the same.
1. An anti-NSP4 antibody comprising a heavy chain and a light chain, wherein the light chain comprises an HVR-L1 comprising the sequence of SEQ ID NO:19, an HVR-L2 comprising the sequence of SEQ ID NO:11, and an HVR-L3 comprising the sequence of SEQ ID NO:12; and wherein the heavy chain comprises an HVR-H1 comprising the sequence of SEQ ID NO:56, an HVR-H2 comprising the sequence of SEQ ID NO:57, and an HVR-H3 comprising the sequence of SEQ ID NO:58.
2. The antibody of claim 1 , wherein the light chain comprises a light chain variable region comprising the sequence of SEQ ID NO:16.
3. The antibody of claim 1 , wherein the heavy chain comprises a heavy chain variable region comprising the sequence of SEQ ID NO: 80.
4. The antibody of claim 1 , wherein the antibody is a monoclonal antibody.
5. The antibody of claim 1 , wherein the antibody is an antibody fragment selected from the group consisting of a Fab, Fab′-SH, Fv, scFv, and (Fab′) 2 fragment.
6. The antibody of claim 1 , wherein the antibody comprises a constant region of human IgG1, IgG2, IgG3, or IgG4.
7. A pharmaceutical composition comprising the anti-NSP4 antibody of claim 1 and a pharmaceutically acceptable carrier.
8. An anti-NSP4 antibody comprising a heavy chain and a light chain, wherein the light chain comprises an HVR-L1 comprising the sequence of SEQ ID NO:19, an HVR-L2 comprising the sequence of SEQ ID NO:11, and an HVR-L3 comprising the sequence of SEQ ID NO:12; and wherein the heavy chain comprises an HVR-H1 comprising the sequence of SEQ ID NO:32, an HVR-H2 comprising the sequence of SEQ ID NO:33, and an HVR-H3 comprising the sequence of SEQ ID NO:34.
9. An anti-NSP4 antibody comprising a heavy chain and a light chain, wherein the light chain comprises an HVR-L1 comprising the sequence of SEQ ID NO:19, an HVR-L2 comprising the sequence of SEQ ID NO:11, and an HVR-L3 comprising the sequence of SEQ ID NO:12; and wherein the heavy chain comprises an HVR-H1 comprising the sequence of SEQ ID NO:38, an HVR-H2 comprising the sequence of SEQ ID NO:39, and an HVR-H3 comprising the sequence of SEQ ID NO:40.
10. An anti-NSP4 antibody comprising a heavy chain and a light chain, wherein the light chain comprises an HVR-L1 comprising the sequence of SEQ ID NO:19, an HVR-L2 comprising the sequence of SEQ ID NO:11, and an HVR-L3 comprising the sequence of SEQ ID NO:12; and wherein the heavy chain comprises an HVR-H1 comprising the sequence of SEQ ID NO:41, an HVR-H2 comprising the sequence of SEQ ID NO:42, and an HVR-H3 comprising the sequence of SEQ ID NO:43.
11. An anti-NSP4 antibody comprising a heavy chain and a light chain, wherein the light chain comprises an HVR-L1 comprising the sequence of SEQ ID NO:19, an HVR-L2 comprising the sequence of SEQ ID NO:11, and an HVR-L3 comprising the sequence of SEQ ID NO:12; and wherein the heavy chain comprises an HVR-H1 comprising the sequence of SEQ ID NO:53, an HVR-H2 comprising the sequence of SEQ ID NO:54, and an HVR-H3 comprising the sequence of SEQ ID NO:55.
12. An anti-NSP4 antibody comprising a heavy chain and a light chain, wherein the light chain comprises an HVR-L1 comprising the sequence of SEQ ID NO:19, an HVR-L2 comprising the sequence of SEQ ID NO:11, and an HVR-L3 comprising the sequence of SEQ ID NO:12; and wherein the heavy chain comprises an HVR-H1 comprising the sequence of SEQ ID NO:59, an HVR-H2 comprising the sequence of SEQ ID NO:60, and an HVR-H3 comprising the sequence of SEQ ID NO:61.