Methods for treating and diagnosing blinding eye diseases
This invention relates to, in part, methods and compositions that are useful for the diagnosis, treatment, or prevention of a blinding eye disease, including in the discovery of drugs that are efficacious against these diseases. Diseases include, for example, age related macular degeneration and reticular pseudodrusen disease, and the methods described herein include, for example, the method named delayed near infrared analysis (DNIRA).
1. A method for evaluating a subject for a dry age-related macular degeneration (AMD) or reticular pseudodrusen (RPD), comprising:
(a) administering a fluorescent compound ingested by retinal pigment epithelial (RPE) cells in the subject's eye, wherein the fluorescent compound is indocyanine green (ICG), which is administered systemically to the subject;
(b) exposing the eye to light having a wavelength of about 600 nm to about 900 nm, wherein the exposing occurs at least 24 hours after administering the fluorescent compound; and
(c) detecting a fluorescent pattern in the subject's eye, wherein the fluorescence pattern is indicative of dry AMD or RPD.
2. The method of claim 1 , wherein the exposing and detecting occurs without re-administration of ICG.
3. The method of claim 1 , wherein the fluorescence occurs in RPE cells.
4. The method of claim 1 , wherein the exposing occurs about 24 hours after administering the fluorescent compound.
5. The method of claim 1 , wherein the exposing occurs about 48 hours after administering the fluorescent compound.
6. The method of claim 1 , wherein the exposing occurs about 72 hours after administering the fluorescent compound.
7. The method of claim 1 , wherein the subject is evaluated for dry age-related macular degeneration (AMD).
8. The method of claim 1 , wherein the subject is a mouse, rat, zebrafish, rabbit, non-human primate or human.
9. The method of claim 1 , wherein the method employs performing confocal scanning laser ophthalmoscopy (cSLO), fundus autofluorescence (FAF), or optical coherence tomography (OCT).
10. The method of claim 1 , wherein the presence of dry age-related macular degeneration (AMD) or reticular pseudodrusen (RPD) is indicated by patterns of FAF within patches of RPE damage or loss or outer retinal loss and the patterns are one or more of curvilinear, ribbon-like, reticular, oval, circular, scalloped, halo, and target-like lesions.
11. The method of claim 1 , wherein the presence of dry age-related macular degeneration (AMD) or reticular pseudodrusen (RPD) is indicated by patterns of FAF within a border of patches of RPE damage or loss or outer retinal loss and the patterns are one or more of curvilinear, ribbon-like, reticular, oval, circular, scalloped, halo, and target-like lesions.
12. The method of claim 1 , wherein the presence of dry age-related macular degeneration (AMD) or reticular pseudodrusen (RPD) is indicated by cross-sectional patterns or transverse patterns of RPE and/or outer retinal loss or mounds, triangles, peaks or spikes found in the sub-retinal space as observed with OCT.
13. The method of claim 1 , wherein the method does not comprise administering (i) an additional amount of the fluorescent compound to the subject or (ii) a second fluorescent compound.
14. The method of claim 1 , wherein the subject is human.
15. A method for evaluating a human patient for dry age-related macular degeneration (AMD) or reticular pseudodrusen (RPD), comprising:
(a) administering a fluorescent compound ingested by retinal pigment epithelial (RPE) cells in the human patient's eye, wherein the fluorescent compound is indocyanine green (ICG) which is administered systemically to the patient;
(b) exposing the eye to light having a wavelength of about 600 nm to about 900 nm, wherein the exposing occurs at least 24 hours after administering the fluorescent compound; and
(c) detecting a fluorescent pattern in the patient's eye, wherein the fluorescence pattern is indicative of dry AMD or RPD.