Peptide having osteoblast proliferation activity and periodontal ligament fibroblast proliferation activity, and use of same
A peptide comprising an amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 2, according to the present invention, shows proliferation and differentiation promotion of osteoblast and proliferation and activation promotion of periodontal ligament fibroblast. A peptide, according to the present invention, increases BMP signaling such as SMAD1/5/8 phosphorylation and increases the growth of osteoblast and the expression of a differentiation marker such as COL1A1, BSP and ALP, thereby ultimately showing osteoblast activation. A peptide, according to the present invention, promotes the growth of periodontal ligament fibroblast by means of PI3K and Akt phosphorylation and increases the expression of an activation marker such as COL1A1 and DSPP, thereby ultimately showing periodontal tissue regeneration activation. Provided are a composition for preventing or treating bone diseases and a composition for preventing or treating periodontal diseases, the compositions comprising the aforementioned peptide.
1. A peptide consisting of one amino acid sequence selected from the group consisting of the amino acid sequence of SEQ ID NO: 1 and the amino acid sequence of SEQ ID NO: 2, wherein the N- or C-terminus of the peptide is optionally modified by the presence of a protecting group.
2. The peptide of claim 1 , wherein the peptide promotes the proliferation of osteoblasts.
3. The peptide of claim 1 , wherein the peptide increases the phosphorylation of Smad1, Smad5, and Smad8, or PI3K and Akt.
4. The peptide of claim 1 , wherein the peptide increases the expression of alkaline phosphatase (ALP), collagen type I alpha 1 (COL1A1), and bone sialoprotein (BSP).
5. The peptide of claim 1 , wherein the peptide promotes the proliferation of periodontal ligament fibroblasts.
6. The peptide of claim 1 , wherein the peptide increases the expression of collagen type I alpha 1 (COL1A1) and dentin sialophosphoprotein (DSPP).
7. A method for treating a bone diseases that would benefit from increased osteoblast proliferation, the method comprising administering a composition containing the peptide of claim 1 as an active ingredient to a subject suffering from the bone disease.
8. A method for treating a periodontal diseases that would benefit from increased periodontal ligament proliferation, the method comprising administering a composition containing the peptide of claim 1 as an active ingredient to a subject suffering from the periodontal disease.
9. The peptide of claim 1 , wherein the peptide consists of the amino acid sequence of SEQ ID NO: 1.
10. The peptide of claim 1 , wherein the peptide consists of the amino acid sequence of SEQ ID NO: 2.
11. The method of claim 7 , wherein the composition comprises a peptide consisting of SEQ ID NO: 1.
12. The method of claim 7 , wherein the composition comprises a peptide consisting of SEQ ID NO: 2.
13. The method of claim 8 , wherein the composition comprises a peptide consisting of SEQ ID NO: 1.
14. The method of claim 8 , wherein the composition comprises a peptide consisting of SEQ ID NO: 2.
15. The peptide of claim 1 , wherein the N- or C-terminus of the peptide is modified by the presence of a protecting group.
16. The peptide of claim 15 , wherein the protecting group is selected from the group consisting of an acetyl group, a fluorenyl methoxy carbonyl group, a formyl group, a palmitoyl group, a myristyl group, a stearyl group, and polyethylene glycol (PEG).