IP Library › Granted Patent US 10,006,042
Granted Patent B2
US 10,006,042 · App. 14/904,758 · Granted Jun 26, 2018

Isolated polynucleotides and polypeptides, and methods of using same for increasing plant yield and/or agricultural characteristics

Inventors: Lidor Gil (Rehovot, IL); Ronit Rimon Knopf (Modiin, IL); Noa Matarasso (Tel-Aviv, IL); David Panik (Rehovot, IL); Limor Poraty-Gavra (Holon, IL); Ehud Katz (Tel-Aviv, IL); Hagai Karchi (Moshav Sitriya, IL)
Assignee: Evogene Ltd.
C12N15/8261C07K14/415C12N15/8241C12N15/8243C12N15/8247C12N15/8271C12N15/8273Y02A40/146
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Quick Facts
Patent No.
US 10,006,042
App. No.
14/904,758
Granted
Jun 26, 2018
Kind
B2
Abstract

Provided are isolated polypeptides which are at least 80% homologous to SEQ ID NOs: 710-1153 and 9276-15726, isolated polynucleotides which are at least 80% identical to SEQ ID NOs: 1-709 and 1157-9275, nucleic acid constructs comprising same, transgenic cells expressing same, transgenic plants expressing same and method of using same for increasing yield, abiotic stress tolerance, growth rate, biomass, vigor, oil content, photosynthetic capacity, seed yield, fiber yield, fiber quality, fiber length, and/or nitrogen use efficiency of a plant.

Claims (10)

1. A method of increasing growth rate, biomass, seed yield, and/or photosynthetic capacity of a plant, comprising:

(a) transforming plants with a nucleic acid construct comprising an exogenous polynucleotide comprising a nucleic acid sequence encoding a polypeptide having an amino acid sequence which is at least 95% identical to the amino acid sequence as set forth in SEQ ID NOs: 738, wherein said exogenous polynucleotide is operably linked to a heterologous promoter for directing transcription of said polynucleotide in said plants; and

(b) selecting a transgenic plant obtained from step (a) transformed with said nucleic acid construct which overexpresses said polypeptide, and exhibits increased growth rate, biomass, seed yield, and/or photosynthetic capacity as compared to a wild type plant of the same species which is grown under the same growth conditions.

2. A method of producing a crop of plants comprising growing a crop plant transformed with a nucleic acid construct comprising an exogenous polynucleotide comprising a nucleic acid sequence encoding a polypeptide having an amino acid sequence which is at least 95% identical to the amino acid sequence as set forth in SEQ ID NO: 738, wherein said exogenous polynucleotide is operably linked to a heterologous promoter, wherein the crop plant is obtained from plants which have been transformed with said nucleic acid construct which overexpresses said polypeptide in said plants, and which have been selected for increased growth rate, increased biomass, increased seed yield, and/or increased photosynthetic capacity as compared to a wild type plant of the same species which is grown under the same growth conditions, and thereby producing the crop of plants.

3. The method of claim 1 , wherein said exogenous polynucleotide comprises the nucleic acid sequence selected from the group consisting of SEQ ID NOs: 420 and 29.

4. The method of claim 1 , wherein said polypeptide has the amino acid sequence of the SEQ ID NO: 738.

5. The method of claim 2 , wherein said nucleic acid sequence encodes the amino acid sequence as set forth in SEQ ID NO: 738.

6. The method of claim 2 , wherein said nucleic acid sequence is selected from the group consisting of SEQ ID NOs: 420 and 29.

7. A method of growing a crop, the method comprising seeding seeds and/or planting plantlets of a plant transformed with a nucleic acid construct comprising an exogenous polynucleotide comprising a nucleic acid sequence encoding a polypeptide having at least 95% amino acid sequence identity to SEQ ID NO: 738, wherein said polynucleotide is operably linked to a heterologous promoter, wherein the transformed plant is obtained from plants which have been transformed with said exogenous polynucleotide and which have been selected for at least one trait selected from the group consisting of: increased biomass, increased growth rate, increased seed yield, and increased photosynthetic capacity as compared to a wild type plant of the same species thereby growing the crop and wherein said seeds and said plantlets comprise said nucleic acid construct.

8. The method of claim 1 , wherein said selecting is performed under non-stress conditions.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Feb 10, 2016
From: GIL, LIDOR; RIMON KNOPF, RONIT; MATARASSO, NOA; PANIK, DAVID; PORATY-GAVRA, LIMOR; KATZ, EHUD; KARCHI, HAGAI
To: EVOGENE LTD.
Reel/Frame 037696/0974 →
Continuity (3)
Provisional Application 61932373 · Jan 28, 2014
Provisional Application 61870380 · Aug 27, 2013
Related Publication 20160272987A1 · Sep 22, 2016