Number of IL4 and/or IL13 secreting T-cells as a biomarker for allergic diseases
The present invention relates to an in vitro use of a number of IL-4 and/or IL-13 secreting T-cells as a biomarker for diagnosing and/or monitoring an IgE-dependent allergic disease.
1. An in vitro method for measuring a number of IL-4 and/or IL-13 secreting memory T-cells contained in an isolated biological sample comprising memory T-cells, wherein said isolated biological sample is obtained from an individual having an IgE-dependent allergic disease selected from the group consisting of airborne allergies, food allergies and insect allergies, said method comprising at least the steps of:
(a) contacting for a period of time comprised from about 12 to about 24 h, with an allergen capable of causing said IgE-dependent allergic disease, said isolated biological sample from said individual, in conditions suitable for said memory T-cells to secrete IL-4 and/or IL-13; and
(b) measuring a number of IL-4 and/or IL-13 secreting memory T-cells contained in said biological sample.
2. The method according to claim 1 wherein step (a) is performed for a period of from about 15 h to about 22 h.
3. The method according to claim 1 , further comprising a step of comparing said measured number of memory T cells with a reference value.
4. The method according to claim 1 , wherein a number of memory T-cells secreting IL-4 and a number of memory T-cells secreting IL-13 are measured.
5. The method according to claim 1 , wherein said measuring of the number of IL-4 and/or IL-13 secreting memory T cells is carried out by ELISpot.
6. A method for diagnosing and treating an IgE-dependent allergic disease to an allergen, in an individual suspected of having an IgE-dependent allergic disease, said method comprising at least the steps of:
(a) contacting for a period of time comprised from about 12 to about 24 h, with an allergen capable of causing said IgE-dependent allergic disease, an isolated biological sample from said individual, said sample comprising memory T-cells, in conditions suitable for said memory T-cells to secrete IL-4 and/or IL-13;
(b) measuring a number of IL-4 and/or IL-13 secreting memory T-cells contained in said biological sample;
(c) comparing said measured number of memory T-cells with a reference value;
(d) determining said individual has said IgE-dependent allergic disease when the measured number of memory T-cells is higher than said reference value; and
(e) treating said individual determined in step (d) to have said IgE-dependent allergic disease with an immunotherapy.
7. The method according to claim 6 , wherein step (a) is performed for a period of from about 15 h to about 22 h.
8. The method according to claim 6 , wherein a number of memory T-cells secreting IL-4 and a number of memory T-cells secreting IL-13 are measured.
9. The method according to claim 6 , wherein said measuring of the number of IL-4 and/or IL-13 secreting memory T-cells is carried out by ELISpot.
10. The method according to claim 6 , wherein said IgE-dependent allergic disease is selected from the group consisting of airborne allergies, food allergies and insect allergies.