Chemical arrays and methods of using the same
Methods and compositions for generating mixtures of product molecules from an initial chemical array are provided. In the subject methods, a chemical array of surface immobilized first moieties is subjected to cleavage conditions such that a composition of solution phase first moieties is produced. The resultant composition of solution phase first moieties is then contacted with one or more reactants to produce a mixture of product molecules that are different from the first moieties. Also provided are the arrays employed in the subject methods and kits for practicing the subject methods.
1. A method comprising:
(a) subjecting an array of a plurality of non-nucleic acid polymer features comprising non-nucleic acid polymer first moieties immobilized on a surface of a solid support via a cleavable domain to conditions sufficient to cleave said cleavable domain and generate a solution phase composition of said non-nucleic acid polymer first moieties, wherein said non-nucleic acid features are arranged on the surface of said solid support in addressable regions and wherein said solution phase composition is a mixture comprising two or more of said non-nucleic acid polymer-first moieties; and
(b) contacting said solution phase composition of said non-nucleic acid polymer-first moieties with one or more reactants to produce a mixture of product molecules that are different from said non-nucleic acid polymer first moieties.
2. The method according to claim 1 , wherein said cleavable domain is a base labile domain.
3. The method according to claim 1 , wherein said cleavable domain is an acid labile domain.
4. The method according to claim 1 , wherein said cleavable domain is a photocleavable domain.
5. The method according to claim 1 , wherein said cleavable domain is a chemically cleavable domain.
6. The method according to claim 1 , wherein said cleavable domain is a nucleophile cleavable domain.
7. The method according to claim 1 , wherein said one or more reactants comprises an enzymatic activity.
8. The method according to claim 1 , wherein said mixture comprises at least 25,000 different product molecules.
9. The method according to claim 1 , wherein said product molecules are polysaccharides.
10. The method according to claim 1 , wherein said surface immobilized first moieties are described by the formula:
surface-L-V
wherein:
L is a cleavable domain; and
V is a variable domain;
wherein said variable domain V of said surface immobilized first moieties differs between features.