Fish vaccine
The present document discloses a new species of Aliivibrio , herein denoted Aliivibrio friggiae . A strain Aliivibrio friggiae , denoted Aliivibrio sp. 130206K7F2 506, has been deposited at the National Collection of Industrial and Marine Bacteria and has been assigned accession number NCIMB 42181. Further disclosed is the medical use of Aliivibrio friggiae for vaccinating fish against a new disease identified and herein denoted friggiosis.
1. A composition comprising inactivated bacteria of the species Aliivibrio friggiae , wherein said Aliivibrio friggiae exhibits one or more characteristics selected from the group consisting of not exhibiting haemolytic properties when grown on blood agar, exhibiting degradation of gelatin and nitrate, not exhibiting degradation of tryptophan, and exhibiting inhibited growth in the presence of Aliivibrio wodanis.
2. The composition according to claim 1 , wherein said Aliivibrio friggiae is Aliivibrio sp. 130206K7F2 506, which has been deposited at National Collection of Industrial, Food, and Marine Bacteria and has been assigned accession number NCIMB 42181.
3. The composition according to claim 1 , wherein said composition further comprises a pharmaceutically acceptable excipient and/or adjuvant.
4. The composition according to claim 1 , wherein said composition further comprises bacteria of one or more other genera and/or species and/or strains and/or an antigen(s) of such bacteria.
5. The composition according to claim 4 , wherein said bacteria of one or more other genera and/or species and/or strains are selected from the group consisting of Bizionia sp, Moritella viscosa, Aliivibrio wodanis, Tenacibaculum sp, Tenacibaculum maritimum, Vibrio sp, Photobacter sp, Aeromonas salmonicida ss salmonicida, Aliivibrio logei, Aliivibrio salmonicida, Flavobacterium, Flavobacterium psychrophilum, Flavobacterium columnare , and/or Vibrio anguillarum.
6. The composition according to claim 1 , wherein said composition further comprises Bizionia sp, and/or an antigen(s) thereof.
7. The composition according to claim 1 , wherein said composition further comprises Aliivibrio wodanis and/or an antigen(s) thereof.
8. The composition according to claim 1 , wherein said composition further Aliivibrio wodanis , and/or an antigen(s) thereof, and Bizionia sp, and/or an antigen(s) thereof.
9. The composition according to claim 1 , wherein said composition further comprises Moritella viscosa.
10. A method for preparing the composition of claim 1 , said method comprising the steps of:
a) cultivating bacteria of Aliivibrio friggiae as defined in claim 1 alone or in combination with other bacteria;
b) optionally separating said bacteria from their growth medium;
c) inactivating said bacteria;
d) optionally mixing said bacteria with a pharmaceutically acceptable excipient and/or adjuvant.
11. The method according to claim 10 , wherein said bacteria of Aliivibrio friggiae are cultivated together with one or more bacterium selected from the group consisting of Bizionia sp, Moritella viscosa, Aliivibrio wodanis, Tenacibaculum sp, Tenacibaculum maritimum, Vibrio sp, Photobacter sp, Aeromonas salmonicida ss salmonicida, Aliivibrio logei, Aliivibrio salmonicida, Flavobacterium, Flavobacterium psychrophilum, Flavobacterium columnare , and/or Vibrio anguillarum.
12. The method according to claim 10 , wherein said Aliivibrio friggiae are cultivated together with Aliivibrio wodanis.
13. The method according to claim 12 , wherein said Aliivibrio friggiae and Aliivibrio wodanis are cultivated at about 0.9% NaCl.
14. The method according to claim 1 , wherein said bacteria are inactivated by heat, attenuation, or formalin.
15. A method for treating and/or preventing friggiosis comprising the administration of a pharmaceutically effective amount of a composition as defined in claim 1 to a subject in need thereof.
16. The method of claim 15 , wherein said method further comprises the administration of one or more additional bacteria selected from the group consisting of Bizionia sp, and/or an antigen(s) thereof and Aliivibrio wodanis , and/or an antigen thereof, to a subject in need thereof.
17. The method of claim 15 , wherein said administration further treats and/or prevents bizioniosis and/or wodanosis.
18. The method according to claim 15 , wherein said administration is performed by intraperitoneal injection, bath vaccination and/or by oral vaccination.
19. A biologically pure strain of Aliivibrio sp. 130206K7F2 506, which has been deposited at National Collection of Industrial, Food, and Marine Bacteria and has been assigned accession number NCIMB 42181, wherein said Aliivibrio sp. 130206K7F2 506 is inactivated.