Non-caloric sweeteners and methods for synthesizing
Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebausoside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W).
1. A method of producing synthetic rebaudioside M, the method comprising:
incubating an in vitro reaction mixture comprising rebaudioside D, uridine diphosphate-glucose (UDP-glucose), and a UGT76G1 UDP-glycosyltransferase-sucrose synthase fusion enzyme comprising the amino acid sequence of SEQ ID NO: 9 for a sufficient time to completely convert the rebaudioside D to synthetic rebaudioside M.
2. The method of claim 1 , wherein the in vitro reaction mixture is incubated for at least 24 hours.
3. The method of claim 1 , wherein the method further comprises purifying the synthetic rebaudioside M.
4. The method of claim 3 , wherein the synthetic rebaudioside M is purified using high-performance liquid chromatography (HPLC).