Systems, devices, and methods for agglutination assays using sedimentation
Embodiments of the present invention include methods for conducting agglutination assays using sedimentation. Aggregates may be exposed to sedimentation forces and travel through a density medium to a detection area. Microfluidic devices, such as microfluidic disks, are described for conducting the agglutination assays, as are systems for conducting the assays.
1. A microfluidic device for conducting an agglutination assay, the microfluidic device comprising:
a substrate at least partially defining or supporting:
a mixing chamber comprising a mixture, wherein the mixture includes a sample and a population of coated particles having a density, wherein the population of coated particles include affinity reagents for an analyte of interest, wherein the population of coated particles forms aggregates with the analyte of interest when present in the mixture;
a reservoir containing a density medium, wherein the density medium has a minimum density that is greater than the density of the coated particles;
wherein the mixing chamber and the reservoir are in fluid communication with each other such that the mixture becomes layered on the density medium in the reservoir, and further wherein the aggregates, if formed, travel through the density medium in the reservoir responsive to sedimentation forces.
2. The microfluidic device of claim 1 , wherein the substrate comprises a disk configured to rotate to generate the sedimentation forces.
3. The microfluidic device of claim 1 , wherein the affinity reagents bind the analyte of interest at multiple locations.
4. The microfluidic device of claim 1 , further comprising a valve between the mixing chamber and the reservoir.
5. The microfluidic device of claim 1 , further comprising a detection area in fluid communication with the reservoir, wherein the aggregates, if formed, are collected in the detection area responsive to the sedimentation forces.
6. The microfluidic device of claim 1 , wherein the substrate at least partially defines a plurality of assay areas for conducting parallel assays.