Parvovirus Vp1 unique region polypeptides and compositions thereof
The present invention is directed to mutant parvovirus VP1 unique region polypeptides, compositions comprising such polypeptides, methods of making such compositions, as well as methods for identifying the likely presence of parvovirus-neutralizing antibodies, and methods for assessing the functional immunogenicity of parvovirus vaccines and measuring a correlate of efficacy to assess a treatment for parvovirus infection.
1. A polypeptide comprising a parvovirus VP1 unique region selected from SEQ ID NO: 1, SEQ ID NO: 4, SEQ ID NO:5 and SEQ ID NO:6, wherein the sequence of amino acids at positions 167-171 of said parvovirus unique region has at least two amino acid mutations, where each of said mutations is an amino acid substitution or an amino acid deletion, and wherein the parvovirus VP1 unique region does not cross-react with antibodies that bind parvovirus VP2.
2. An immunogenic composition comprising the polypeptide of claim 1 and a pharmaceutically acceptable carrier.
3. A method of raising an immune response to parvovirus in a subject comprising administering the immunogenic composition of claim 2 to the subject.
4. The polypeptide of claim 1 , wherein the sequence of amino acids corresponding to positions 167-171 of SEQ ID NO:1 has at least three amino acid substitutions or deletions.
5. The polypeptide of claim 1 , wherein the sequence of amino acids corresponding to positions 167-171 of SEQ ID NO:1 has at least four amino acid substitutions or deletions.
6. The polypeptide of claim 1 , which does not include a Y168F or H170Y substitution.