Methods for inducing glucose uptake
The present invention provides a method for inducing glucose uptake in a muscle cell by inhibiting Trim32 protein in the cell and to a method for inducing glucose uptake in a muscle cell, by increasing the abundance of plakoglobin protein in the cell.
1. A method for inducing glucose uptake in a muscle cell in a subject suffering from insulin resistance, comprising the step of inhibiting Trim32 protein in said cell in the subject suffering from insulin resistance, thereby inducing glucose uptake in a muscle cell in the subject suffering from insulin resistance.
2. The method of claim 1 , wherein said muscle cell is a skeletal muscle cell.
3. The method of claim 1 , wherein said inhibiting Trim32 in said cell is further inducing muscle fiber growth.
4. The method of claim 1 , wherein said inhibiting Trim32 in said cell is inducing the phosphorylation of insulin receptor in said cell.
5. The method of claim 1 , wherein said inhibiting Trim32 is inducing the accumulation of plakoglobin.
6. The method of claim 1 , wherein said inhibiting Trim32 in said cell is expressing a dominant negative Trim 32 protein in said cell.
7. The method of claim 6 , wherein said expressing a dominant negative Trim 32 protein in said cell is inserting a synthetic mRNA molecule encoding said dominant negative Trim 32 protein into said cell, said synthetic mRNA molecule comprises a 5-methylcytidine base, a pseudouridine base, or a combination thereof.
8. The method of claim 6 , wherein said expressing a dominant negative Trim 32 protein in said cell is delivering a nucleic acid molecule encoding said dominant negative Trim 32 protein via electroporation into said cell.
9. The method of claim 6 , wherein said expressing a dominant negative Trim 32 protein in said cell is delivering a nucleic acid molecule encoding said dominant negative Trim 32 protein via a cationic vector into said cell.
10. A method for inducing glucose uptake in a muscle cell in a subject suffering from insulin resistance, comprising the step of increasing the abundance of plakoglobin protein in said cell, thereby inducing glucose uptake in a muscle cell in the subject suffering from insulin resistance.
11. The method of claim 10 , wherein said muscle cell is a skeletal muscle cell.
12. The method of claim 10 , wherein said increasing the abundance of plakoglobin protein in said cell further results in inducing muscle fiber growth.
13. The method of claim 10 , wherein said increasing the abundance of plakoglobin protein in said cell results in inducing the phosphorylation of insulin receptor in said cell.
14. The method of claim 10 , wherein said increasing the abundance of plakoglobin protein in said cell is transfecting said cell with a vector comprising a nucleic acid molecule, said nucleic acid molecule encodes said plakoglobin protein.
15. The method of claim 14 , wherein said vector comprises a constitutively active promoter.
16. The method of claim 14 , wherein said nucleic acid molecule is a synthetic mRNA molecule comprising a 5-methylcytidine base, a pseudouridine base, or a combination thereof.
17. The method of claim 14 , wherein said transfecting said cell is via electroporation into said cell.
18. The method of claim 14 , wherein said vector is a cationic vector.