Processes for producing ethanol and yeast
The present invention relates to processes for producing ethanol from starch-containing material by liquefying the starch-containing material at a temperature above the initial gelatinization temperature using an alpha-amylase; saccharifying using a glucoamylase and fermenting using a Saccharomyces cerevisiae yeast strain deposited under Accession No. V14/004037 at National Measurement Institute, Victoria, Australia) or a fermenting organism strain having properties that are about the same as that of the deposited Saccharomyces cerevisiae strain or a derivative of Saccharomyces strain V14/004037 having the defining characteristics of strain V14/004037. The invention also relates to a Saccharomyces yeast strain deposited under the Budapest Treaty and having NMI accession no. V14/004037 or a derivative of strain V14/004037 which exhibits one or more defining characteristics of strain V14/004037. The invention also relates to a process of recovering/extracting oil from an ethanol process of the invention using a Saccharomyces strain of the invention and compositions comprising a Saccharomyces yeast strain of the invention and naturally occurring and/or non-naturally occurring components.
1. A Saccharomyces yeast strain deposited under the Budapest Treaty and having NMI accession no. V14/004037 ( Saccharomyces cerevisiae MBG4851).
2. A composition comprising a Saccharomyces yeast strain of claim 1 , and one or more naturally occurring and/or non-naturally occurring components selected from the group consisting of: surfactants, emulsifiers, gums, swelling agents, and antioxidants.
3. A process for producing ethanol from starch-containing material comprising the steps of:
i) liquefying the starch-containing material at a temperature above the initial gelatinization temperature using an alpha-amylase;
ii) saccharifying using a glucoamylase; and
iii) fermenting using the Saccharomyces yeast strain of claim 1 .
4. The process of claim 3 , wherein 100 to 600 ppm urea is added in saccharification step ii) or fermentation step iii) or added during simultaneous saccharification and fermentation (SSF) of steps ii) and iii).
5. The process of claim 3 , wherein a protease is added in saccharification step ii) or fermentation step iii) or added during simultaneous saccharification and fermentation (SSF) of steps ii) and iii).
6. A method of producing ethanol, comprising incubating the yeast strain of claim 1 with a substrate comprising a fermentable sugar under conditions which permit fermentation of the fermentable sugar to produce ethanol.
7. A method of producing distiller's grain, comprising:
(a) incubating the yeast strain of claim 1 with a substrate comprising fermentable sugar under conditions which allow fermentation of the fermentable sugar to produce ethanol and distiller's grains;
(b) isolating the distiller's grains.
8. A process of recovering/extracting oil from an ethanol fermentation product production process comprising the steps of:
i) liquefying starch-containing material at a temperature above the initial gelatinization temperature using an alpha-amylase;
ii) saccharifying using a glucoamylase;
iii) fermenting using a fermenting organism;
iv) recovering the fermentation product to form whole stillage;
v) separating the whole stillage into thin stillage and wet cake;
vi) optionally concentrating the thin stillage into syrup;
wherein oil is recovered/extracted downstream from fermentation step iii) and wherein the fermenting organism is the Saccharomyces yeast strain of claim 5 .