Electron microscope sample holder for forming a gas or liquid cell with two semiconductor devices
A novel sample holder for specimen support devices for insertion in electron microscopes. The novel sample holder of the invention allows for the introduction of gases or liquids to specimens for in situ imaging, as well as electrical contacts for electrochemical or thermal experiments.
1. A sample holder for an electron microscope, said sample holder comprising a sample holder body and a sample holder lid,
wherein the sample holder body comprises a deep pocket having a deep pocket bottom and deep pocket walls for the positioning of a first microelectronic device therein and a shallow pocket having a shallow pocket bottom and shallow pocket walls for the positioning of a second microelectronic device therein,
wherein the first microelectronic device comprises a first top surface and a first bottom surface, wherein the first top surface comprises at least one first electrical contact and the first bottom surface is positioned in the deep pocket bottom, and the second microelectronic device comprises a second top surface and a second bottom surface,
wherein the second top surface comprises at least one second electrical contact and the second top surface is positioned in the shallow pocket bottom such that at least one electrical connection is made between the first electrical contact and the second electrical contact.
2. The sample holder of claim 1 , wherein the shallow pocket fully encloses the deep pocket.
3. The sample holder of claim 1 , wherein at least one shallow pocket electrical contact is positioned on the shallow pocket bottom.
4. The sample holder of claim 3 , wherein at least one additional electrical connection is made between the at least one shallow pocket electrical contact and the at least one second electrical contact.
5. The sample holder of claim 1 , wherein the first microelectronic device is a thermal device or an electrical biasing device.
6. The sample holder of claim 1 , wherein the second microelectronic device is a window device.
7. The sample holder of claim 1 , wherein the at least one first electrical contact and/or the at least one second electrical contact comprises gold.
8. The sample holder of claim 1 ,
wherein a deep sealing means is positioned on the deep pocket bottom and surrounds a hole such that a seal is formed around the hole when pressure is applied to the first microelectronic device, and
wherein a shallow sealing means is positioned on the shallow pocket bottom and surrounds the first microelectronic device such that when pressure is applied to the second microelectronic device a seal is formed and fluids cannot escape from around the first and second microelectronic devices.
9. The sample holder of claim 1 , wherein the sample holder body and the sample holder lid have an electron beam hole for passage of an electron beam through the sample holder.
10. The sample holder of claim 1 , wherein the sample holder body comprises at least one component selected from the group consisting of:
(a) at least one inlet supply line;
(b) at least one outlet supply line;
(c) at least one sealing means;
(d) securing means for securing the sample holder lid to the sample holder body; and
(e) combinations of (a)-(d) thereof.
11. The sample holder of claim 1 , wherein the sample holder lid comprises at least one component selected from the group consisting of:
(a) at least one sealing means;
(b) securing means for securing the sample holder lid to the sample holder body; and
(c) combinations of (a)-(b) thereof.
12. The sample holder of claim 8 , wherein the deep and/or shallow sealing means comprise an o-ring.
13. The sample holder of claim 10 , wherein the securing means comprise screws.
14. The sample holder of claim 1 , wherein the first and second microelectronic devices are aligned so that an electron beam passes through the sample holder having the microelectronic devices therein.
15. A method of imaging a sample in a liquid and/or gaseous environment in an electron microscope, said method comprising;
inserting a sample in a sample holder,
inserting the sample holder comprising the sample in an electron microscope,
introducing a liquid and/or gas to the sample in the sample holder, and
imaging the sample in the electron microscope,
wherein the sample holder comprises a sample holder body and a sample holder lid, wherein the sample holder body comprises a deep pocket having a deep pocket bottom and deep pocket walls for the positioning of a first microelectronic device therein and a shallow pocket having a shallow pocket bottom and shallow pocket walls for the positioning of a second microelectronic device therein,
wherein the first microelectronic device comprises a first top surface and a first bottom surface, wherein the first top surface comprises at least one first electrical contact and the first bottom surface is positioned in the deep pocket bottom, and the second microelectronic device comprises a second top surface and a second bottom surface,
wherein the second top surface comprises at least one second electrical contact and the second top surface is positioned in the shallow pocket bottom such that at least one electrical connection is made between the first electrical contact and the second electrical contact.
16. The method of claim 15 , wherein the first microelectronic device comprises a heating device or an electrical biasing device.
17. The method of claim 15 , wherein the second microelectronic device comprises a window device.
18. The method of claim 15 , wherein at least one shallow pocket electrical contact is positioned on the shallow pocket bottom.
19. The method of claim 18 , wherein at least one additional electrical connection is made between the at least one shallow pocket electrical contact and the at least one second electrical contact.