Stabilization of pollen production in maize
The present invention provides methods and compositions for producing elite lines of corn exhibiting reduced tassel skeletonization severity (TSS). Also provided in the present invention are corn plants exhibiting reduced TSS resulting from such methods, and methods for breeding corn such that the reduced tassel skeletonization traits may be transferred to a desired genetic background.
1. A method of obtaining a corn plant with reduced tassel skeletonization severity, comprising:
a) providing a population of corn plants;
b) obtaining at least one nucleic acid sample from at least one plant within said population;
c) detecting in the nucleic acid sample the presence of a reduced tassel skeletonization allele comprising SEQ ID NO: 27, wherein N at nucleotide position 162 is a nucleotide that is associated with the presence of the reduced tassel skeletonization severity trait;
d) selecting a corn plant from said population of corn plants based on the presence of the reduced tassel skeletonization allele in the nucleic acid sample; and
e) crossing the corn plant comprising the reduced tassel skeletonization allele with a second corn plant to produce progeny plants, wherein at least one progeny plant comprises the reduced tassel skeletonization allele and exhibits reduced tassel skeletonization severity when compared to a plant lacking said allele.
2. The method of claim 1 , wherein step (a) of providing comprises crossing a first corn plant comprising a reduced tassel skeletonization allele with a second corn plant to produce a population of corn plants.
3. The method of claim 2 , wherein producing the population of corn plants comprises backcrossing.
4. The method of claim 1 , wherein step (c) of detecting comprises the use of an oligonucleotide probe.
5. A method of producing a corn plant with reduced tassel skeletonization severity, comprising:
a) crossing a first corn plant comprising a reduced tassel skeletonization allele with a second corn plant of a different genotype to produce one or more progeny plants; and
b) using marker-assisted selection to select a progeny plant based on the presence of said allele, wherein said allele comprises SEQ ID NO: 27, wherein N at nucleotide position 162 is a nucleotide that is associated with the presence of the reduced tassel skeletonization severity trait;
wherein said allele confers reduced tassel skeletonization severity compared to a plant lacking said allele.
6. The method of claim 5 , further comprising:
c) crossing said progeny plant with itself or a second plant to produce one or more further progeny plants; and
d) selecting a further progeny plant comprising said allele.
7. The method of claim 6 , wherein step (d) of selecting comprises marker-assisted selection.
8. The method of claim 7 , wherein said marker-assisted selection comprises selecting a progeny plant based on the presence of a reduced tassel skeletonization allele, wherein said allele comprises SEQ ID NO: 27 and wherein N at nucleotide position 162 is a nucleotide that is associated with the presence of the reduced tassel skeletonization severity trait.
9. The method of claim 6 , wherein said further progeny plant is an F2-F7 progeny plant.
10. The method of claim 9 , wherein producing the progeny plant comprises backcrossing.
11. The method of claim 10 , wherein backcrossing comprises from 2-7 generations of backcrosses.
12. The method of claim 10 , wherein backcrossing comprises marker-assisted selection.
13. The method of claim 12 , wherein backcrossing comprises marker-assisted selection in at least two generations.
14. The method of claim 13 , wherein backcrossing comprises marker-assisted selection in all generations.
15. The method of claim 12 , wherein marker-assisted selection comprises selecting a progeny plant based on the presence of a reduced tassel skeletonization allele, wherein said allele comprises SEQ ID NO: 27 and wherein N at nucleotide position 162 is a nucleotide that is associated with the presence of the reduced tassel skeletonization severity trait.
16. The method of claim 5 , wherein said first corn plant is an inbred or a hybrid.
17. The method of claim 5 , wherein said second corn plant is an agronomically elite corn plant.
18. The method of claim 17 , wherein said agronomically elite corn plant is an inbred or a hybrid.
19. A method of obtaining a corn plant with reduced tassel skeletonization severity, comprising:
a) providing a population of corn plants;
b) obtaining at least one nucleic acid sample from at least one plant within said population;
c) detecting in the nucleic acid sample the presence of a reduced tassel skeletonization allele, wherein said reduced tassel skeletonization allele comprises at least one of SEQ ID NOs: 1 or 13, wherein N at nucleotide position 453 of SEQ ID NO: 1 or N at nucleotide position 316 of SEQ ID NO: 13 is a nucleotide that is associated with the presence of the reduced tassel skeletonization severity trait;
d) selecting a corn plant from said population of corn plants based on the presence of the reduced tassel skeletonization allele in the nucleic acid sample;
e) crossing the corn plant comprising the reduced tassel skeletonization allele with a second corn plant to produce progeny plants, wherein at least one progeny plant comprises the reduced tassel skeletonization allele and exhibits reduced tassel skeletonization severity when compared to a plant lacking said allele; and
f) phenotypically selecting said at least one progeny plant based on the reduced tassel skeletonization severity trait.
20. The method of claim 19 , wherein step (a) of providing comprises crossing a first corn plant comprising a reduced tassel skeletonization allele with a second corn plant to produce a population of corn plants.
21. The method of claim 20 , wherein producing the population of corn plants comprises backcrossing.
22. The method of claim 19 , wherein step (c) of detecting comprises the use of an oligonucleotide probe.
23. A method of producing a corn plant with reduced tassel skeletonization severity, comprising:
a) crossing a first corn plant with a genotypically different second corn plant to produce one or more progeny plants, wherein the first corn plant phenotypically exhibits the trait of reduced tassel skeletonization severity compared to the second corn plant, and wherein said first corn plant comprises at least one reduced tassel skeletonization severity allele conferring said reduced tassel skeletonization severity; and
b) using marker-assisted selection to select a progeny plant based on the presence of said allele, wherein said allele comprises at least one of SEQ ID NOs: 1 or 13, wherein N at nucleotide position 453 of SEQ ID NO: 1 or N at nucleotide position 316 of SEQ ID NO: 13 is a nucleotide that is associated with the presence of the reduced tassel skeletonization severity trait.
24. The method of claim 23 , further comprising:
c) crossing said progeny plant with itself or a second plant to produce one or more further progeny plants; and
d) selecting a further progeny plant comprising said allele.
25. The method of claim 24 , wherein step (d) of selecting comprises marker-assisted selection.
26. The method of claim 25 , wherein said marker-assisted selection comprises selecting a progeny plant based on the presence of at least one reduced tassel skeletonization allele, wherein said allele comprises at least one of SEQ ID NOs: 1 or 13, wherein N at nucleotide position 453 of SEQ ID NO: 1 or N at nucleotide position 316 of SEQ ID NO: 13 is a nucleotide that is associated with the presence of the reduced tassel skeletonization severity trait.
27. The method of claim 24 , wherein said further progeny plant is an F2-F7 progeny plant.
28. The method of claim 27 , wherein producing the progeny plant comprises backcrossing.
29. The method of claim 28 , wherein backcrossing comprises from 2-7 generations of backcrosses.
30. The method of claim 28 , wherein backcrossing comprises marker-assisted selection.
31. The method of claim 30 , wherein backcrossing comprises marker-assisted selection in at least two generations.
32. The method of claim 31 , wherein backcrossing comprises marker-assisted selection in all generations.
33. The method of claim 30 , wherein marker-assisted selection comprises selecting a progeny plant based on the presence of at least one reduced tassel skeletonization allele, wherein said allele comprises at least one of SEQ ID NOs: 1 or 13, wherein N at nucleotide position 453 of SEQ ID NO: 1 or N at nucleotide position 316 of SEQ ID NO: 13 is a nucleotide that is associated with the presence of the reduced tassel skeletonization severity trait.
34. The method of claim 23 , wherein said first corn plant is an inbred or a hybrid.
35. The method of claim 23 , wherein said second corn plant is an agronomically elite corn plant.
36. The method of claim 35 , wherein said agronomically elite corn plant is an inbred or a hybrid.