Fluid exchange methods and devices
Methods and devices for exchanging therapeutic agents, such as cells, from one liquid medium to another liquid medium are provided. Aspects of embodiments of the methods include transferring a therapeutic agent from a first medium, such as a freezing, storage or shipping buffer, into a second medium, such as a sterile physiologically compatible buffer. In certain aspects, the transfer of the therapeutic agent from a first medium to a second medium involves the use of acoustic-focusing, or acoustophoresis. Embodiments of the subject methods may facilitate the transfer of a therapeutic agent from a storage medium to an infusion medium, and in certain embodiments include administering the therapeutic agent contained in the infusion medium to the subject. Also provided by the present disclosure are devices for practicing the subject methods.
1. A method of preparing a particulate infusion composition, the method comprising:
combining a first liquid comprising a therapeutic agent in a storage medium and present in a container and a second liquid infusion medium in a manner sufficient to produce a laminar flow of the first liquid and second liquid infusion medium; and
acoustophoretically moving the therapeutic agent from a first liquid into the second liquid infusion medium to produce a liquid infusion medium comprising the therapeutic agent.
2. The method according to claim 1 , wherein the method further comprises infusing the second liquid infusion medium comprising the therapeutic agent into a subject.
3. The method according to claim 2 , wherein the subject is human.
4. The method according to claim 2 , wherein the therapeutic agent was obtained from the subject.
5. The method according to claim 1 , wherein the therapeutic agent comprises cells.
6. The method according to claim 1 , wherein the first liquid comprises a cryoprotectant.
7. The method according to claim 1 , wherein combining the first liquid and second liquid infusion medium comprises introducing the first liquid and second liquid infusion medium into a channel of an acoustophoresis device such that the first liquid flows along the sides of the channel and the second liquid infusion medium flows in the center of the channel to produce the laminar flow.
8. The method according to claim 1 , wherein the method is performed under sterile conditions.
9. The method according to claim 1 , wherein the container comprises a freezing bag.
10. The method according to claim 9 , wherein the freezing bag comprises an Ethinyl Vinyl Acetate (EVA) freezing bag.