Method of treating heart tissue using induced pluripotent stem cells
This document provides methods and materials related to induced pluripotent stem cells. For example, induced pluripotent stem cells, compositions containing induced pluripotent stem cells, methods for obtaining induced pluripotent stem cells, and methods for using induced pluripotent stem cells are provided. In addition, methods and materials for using induced pluripotent stem cells to repair tissue (e.g., cardiovascular tissue) in vivo as well as methods and materials for using induced pluripotent stem cells to assess their therapeutic potential in appropriate animal models are provided.
1. A method for treating myocardial infarction of a heart in a mammal, wherein said method comprises:
administering induced pluripotent stem (iPS) cells into a site of said myocardial infarction in said mammal such that (a) said site of myocardial infarction in said mammal is repaired without tumor formation in said mammal from said iPS cells, (b) ejection fraction of said heart is increased, (c) fractional shortening of said heart is increased, and (d) regional septal wall thickness in systole of said heart is increased,
wherein said iPS cells are of the same species as said mammal,
wherein said iPS cells were obtained using:
i) a human Oct3/4 polypeptide, a human Sox2 polypeptide, a human Klf4polypeptide, and a human c-Myc polypeptide; or
ii) one or more non-integrating vectors comprising nucleic acid sequences encoding said human Oct3/4 polypeptide, said human Sox2 polypeptide, said human Klf4polypeptide, and said human c-Myc polypeptide.
2. The method of claim 1 , wherein said iPS cells were obtained using said one or more non-integrating vectors, and wherein said one or more non-integrating vectors further comprise nucleic acid encoding a human Nanog polypeptide.
3. The method of claim 1 , wherein said iPS cells were obtained using said one or more non-integrating vectors, and wherein said one or more non-integrating vectors are non-integrating viral vectors.
4. A method for treating myocardial infarction in a mammal, wherein said method comprises:
administering induced pluripotent stem (iPS) cells into a site of said myocardial infarction in said mammal such that (a) progeny of said iPS cells become engrafted with said site of said myocardial infarction of said mammal without tumor formation in said mammal from said iPS cells, (b) ejection fraction of said heart is increased, (c) fractional shortening of said heart is increased, and (d) regional septal wall thickness in systole of said heart is increased,
wherein said iPS cells are of the same species as said mammal,
wherein said iPS cells were obtained using:
i) a human Oct3/4 polypeptide, a human Sox2 polypeptide, a human Klf4polypeptide, and a human c-Myc polypeptide; or
ii) one or more non-integrating vectors comprising nucleic acid sequences encoding said human Oct3/4 polypeptide, said human Sox2 polypeptide, said human Klf4polypeptide, and said human c-Myc polypeptide.
5. The method of claim 4 , wherein said iPS cells were obtained using said one or more non-integrating vectors, and wherein said one or more non-integrating vectors further comprise nucleic acid encoding a human Nanog polypeptide.
6. The method of claim 4 , wherein said iPS cells were obtained using said one or more non-integrating vectors, and wherein said one or more non-integrating vectors are non-integrating viral vectors.