Colostrum/milk protein compositions
Provided are colostrum and milk polypeptides recombinantly expressed in photosynthetic organisms containing colostrum and milk polypeptides, compositions comprising such organisms and methods for producing such organisms.
1. An isolated nucleus of a photosynthetic organism, wherein the nucleus is transformed with a polynucleotide encoding mammalian milk lactadherin, wherein the lactadherin polypeptide has at least about 90%, 95%, 96%, 97%, 98%, or 99% sequence identity to SEQ ID NO:24.
2. The nucleus of claim 1 , wherein the nucleus is from a microalgae.
3. The nucleus of claim 1 , wherein the photosynthetic organism is selected from the group consisting of Chlorophyta (green algae), Rhodophyta (red algae), Stramenopiles (heterokonts), Xanthophyceae (yellow-green algae), Glaucocystophyceae (glaucocystophytes), Chlorarachniophyceae (chlorarachniophytes), Euglenida (euglenids), Haptophyceae (coccolithophorids), Chrysophyceae (golden algae), Cryptophyta (cryptomonads), Dinophyceae (dinoflagellates), Haptophyceae (coccolithophorids), Bacillariophyta (diatoms), Eustigmatophyceae (eustigmatophytes), Raphidophyceae (raphidophytes), Scenedesmaceae and Phaeophyceae (brown algae).
4. The nucleus of claim 1 , wherein the nucleus is from a Chlorophyta (green algae) nucleus.
5. The nucleus of claim 1 , wherein the photosynthetic organism is selected from the group consisting of Chlamydomonas reinhardtii, Dunaliella salina, Haematococcus pluvialis, Chlorella vulgaris, Acutodesmus obliquus , and Scenedesmus dimorphus.
6. The nucleus of claim 4 , wherein the green alga is selected from the group consisting of Chlamydomonas, Dunaliella, Haematococcus, Chlorella , and Scenedesmaceae.
7. The nucleus of claim 1 , wherein the polynucleotide encoding mammalian milk lactadherin is integrated into the nuclear genome.
8. The nucleus of claim 1 , wherein the mammalian milk lactadherin is from a mammal selected from the group consisting of bovine, ovine and caprine.
9. An isolated photosynthetic cell comprising the nucleus of claim 1 .
10. An isolated cell from a photosynthetic organism, the cell transformed with a polynucleotide encoding mammalian milk lactadherin, wherein the lactadherin polypeptide has at least about 90%, 95%, 96%, 97%, 98%, or 99% sequence identity to SEQ ID NO:24.
11. The cell of claim 10 , wherein the cell is from a cyanobacteria.
12. The cell of claim 10 , wherein the mammalian milk lactadherin is from a mammal selected from the group consisting of bovine, ovine and caprine.
13. The cell of claim 10 , wherein the polynucleotide encoding mammalian milk lactadherin is integrated into the nuclear genome of the cell.
14. The cell of claim 10 , wherein the cell is intact and/or freeze-dried.
15. A photosynthetic organism comprising the cell of claim 10 .
16. A method for producing mammalian milk lactadherin, comprising culturing the cell of claim 10 under conditions sufficient to produce milk lactadherin.
17. A composition edible by a mammal comprising a population of cells of claim 10 .
18. The composition of claim 17 , wherein the composition is selected from a beverage, a food, a feed, a food supplement and a nutraceutical.
19. The composition of claim 17 , wherein the composition is selected from the group consisting of a compressed algal cake, an algal paste and an algal powder.
20. The composition of claim 17 , wherein the composition is freeze-dried, lyophilized or spray-dried.
21. The nucleus of claim 1 , wherein the polynucleotide encoding mammalian milk lactadherin has at least about 90%, 95%, 98%, or 99% sequence identity to SEQ ID NO:23.
22. The nucleus of claim 1 , wherein the nucleus is from a higher plant selected from Brassicaceae, Solanaceae, Phaseoleae, Zea and Oryzeae.
23. The cell of claim 10 , wherein the polynucleotide encoding mammalian milk lactadherin has at least about 90%, 95%, 98%, or 99% sequence identity to SEQ ID NO:23.
24. The cell of claim 10 , wherein the cell is from a microalgae.
25. The cell of claim 10 , wherein the photosynthetic organism is selected from the group consisting of Chlorophyta (green algae), Rhodophyta (red algae), Stramenopiles (heterokonts), Xanthophyceae (yellow-green algae), Glaucocystophyceae (glaucocystophytes), Chlorarachniophyceae (chlorarachniophytes), Euglenida (euglenids), Haptophyceae (coccolithophorids), Chrysophyceae (golden algae), Cryptophyta (cryptomonads), Dinophyceae (dinoflagellates), Haptophyceae (coccolithophorids), Bacillariophyta (diatoms), Eustigmatophyceae (eustigmatophytes), Raphidophyceae (raphidophytes), Scenedesmaceae and Phaeophyceae (brown algae).
26. The cell of claim 10 , wherein the nucleus is from a Chlorophyta (green algae) nucleus.
27. The cell of claim 10 , wherein the green alga is selected from the group consisting of Chlamydomonas, Dunaliella, Haematococcus, Chlorella , and Scenedesmaceae.
28. The cell of claim 10 , wherein the photosynthetic organism is selected from the group consisting of Chlamydomonas reinhardtii, Dunaliella salina, Haematococcus pluvialis, Chlorella vulgaris, Acutodesmus obliquus , and Scenedesmus dimorphus.
29. The cell of claim 10 , wherein the cell is from a higher plant selected from Brassicaceae, Solanaceae, Phaseoleae, Zea and Oryzeae.
30. The cell of claim 10 , wherein the lactadherin polypeptide comprises an amino acid sequence that promotes retention in the endoplasmic reticulum.
31. The cell of claim 10 , wherein the lactadherin polypeptide comprises an amino acid sequence that promotes secretion from the cell.
32. The cell of claim 31 , wherein the amino acid sequence that promotes secretion from the cell comprises SEQ ID NO:45, SEQ ID NO:46 or SEQ ID NO:47.
33. A culture medium comprising a population of cells of claim 31 .
34. The method of claim 16 , further comprising purifying or isolating the milk lactadherin.
35. The method of claim 16 , wherein the milk lactadherin is not purified or isolated.