Microfluidic structure, microfluidic device having the same and method of controlling the microfluidic device
A microfluidic structure in which a plurality of chambers arranged at different positions are connected in parallel and into which a fixed amount of fluid may be efficiently distributed without using a separate driving source, and a microfluidic device having the same. The microfluidic device includes a platform having a center of rotation and including at least one microfluidic structure. The microfluidic structure includes a sample supply chamber configured to accommodate a sample, a plurality of first chambers arranged in a circumferential direction of the platform at different distances from the center of rotation of the platform, and a plurality of siphon channels, each of the siphon channels being connected to a corresponding one of the first chambers.
1. A method of controlling a test device including a microfluidic device including a platform provided with an accommodating chamber configured to accommodate a fluid, a metering chamber configured to meter the amount of the fluid, a reaction chamber directly adjoining the metering chamber and configured to have a chromatographic reaction to occur therein using the fluid metered in the metering chamber and introduced thereinto, and a channel to connect the accommodating chamber and the metering chamber to each other, the method comprising:
rotating the platform and transferring the fluid accommodated in the accommodating chamber to the metering chamber; and
repeating intervals comprising increasing rotational speed of the platform and stopping rotation thereof, such that the fluid flows from the metering chamber directly into the reaction chamber.
2. A method of controlling a test device including a microfluidic device including a platform provided with an accommodating chamber configured to accommodate a fluid, a metering chamber configured to meter the amount of the fluid, a reaction chamber configured to have a chromatographic reaction to occur therein using the fluid metered in the metering chamber and introduced thereinto, and a channel to connect the accommodating chamber, the metering chamber and the reaction chamber to each other, the method comprising:
rotating the platform and transferring the fluid accommodated in the accommodating chamber to the metering chamber; and
repeating intervals comprising increasing rotational speed of the platform and stopping rotation thereof, such that the fluid flows into the reaction chamber, and further comprising, upon transferring the fluid to the metering chamber, stopping the platform such that a first order reaction occurs between the fluid and a marker conjugate accommodated in the metering chamber.
3. The method according to claim 2 , further comprising, upon introduction of the fluid into the reaction chamber, stopping the platform.
4. The method according to claim 3 , further comprising, when the platform is stopped, absorbing the fluid in a detection region provided in the reaction chamber, and transferring the fluid remaining in the metering chamber to the reaction chamber.
5. The method according to claim 1 , further comprising, upon completion of the chromatographic reaction in the reaction chamber, rotating the platform to remove the fluid remaining in the reaction chamber.
6. The method of claim 1 , wherein the chromatographic reaction comprises chromatography based on capillary pressure.
7. The method of claim 1 , wherein the repeating intervals moves the fluid from the metering chamber into the reaction chamber as a result of the repeating intervals comprising increasing rotational speed and the stopping rotation.
8. The method of claim 7 , wherein the repeating intervals comprises rotation in a single direction.
9. The method of claim 1 , wherein the platform has a center of rotation, and the metering chamber and the reaction chamber are located at a common radial distance from the center of rotation of the platform, and wherein the fluid flows into the reaction chamber from the metering chamber in a circumferential direction of the platform as a result of the repeating intervals comprising increasing rotational speed and the stopping rotation.