Treating a bacterial skin infection with a whole, leech saliva extract
Methods are also provided for isolating and using a whole-saliva leech extract in the treatment of a bacterial skin infection. The methods can include feeding a phagostimulatory agent to a leech; inducing a regurgitation in the leech, the inducing including placing the leech in an environment having a temperature of less than about 0° C.; and, collecting an unrefined, whole saliva in the regurgitation of the cooled leech. The methods can include revitalizing the leech by warming it at a temperature ranging from about 5° C. to about 40° C. Stable, lyophilized, whole-saliva extracts of a leech are also provided, the extract having a stable activity when stored for use at a temperature below about −20° C., the extract maintaining at least 70% of the activity for at least 6 months. The extracts can be used to treat solid tumors, treat liquid tumors, treat diabetes, treat a viral disease, treat a parasitic disease, treat an antibacterial disease, or serve as an anti-oxidant.
1. A method of creating a dosage form of a lyophilized, whole saliva extract of a leech for topical administration to a subject, the method comprising:
feeding a phagostimulatory agent to a leech;
inducing regurgitation in the leech, the inducing including placing the leech in an environment having a temperature ranging from about −5° C. to about 15° C.,
collecting an unrefined, whole saliva in the regurgitation of the cooled leech;
removing solid components from the unrefined, whole saliva to create a refined, whole saliva;
lyophilizing separate volumes of the refined, whole saliva extract, the volumes not exceeding about 2 ml each; and,
formulating the lyophilized volumes into a dosage form for topical administration, the formulating including selecting a pharmaceutically acceptable carrier for the topical administration of the lyophilized volumes to the subject.
2. The method of claim 1 , wherein the formulating includes creating a lotion, a cream, an ointment, a spray, or an emulsion.