Compositions and methods to modulate cell activity
The present invention provides methods and compositions for the remote control of cell function based on the use of radiofrequency waves to excite nanoparticles targeted to specific cell types. The nanoparticles may be applied to the target cell extracellularly and/or expressed intracellularly. The cell type of interest expresses a temperature sensitive channel wherein excitation of the nanoparticles results in a localized temperature increase that is transduced into a cellular response. Such cellular responses may include, for example, increases in gene expression resulting in production of one or more physiologically active proteins. The expression of such proteins can be used to treat a variety of different inherited or acquired diseases or disorders in a subject. Accordingly, the invention provides a generic approach for treatment of any disease associated with a protein deficiency.
1. A cell type of interest comprising nanoparticles that are selective for a temperature sensitive channel or receptor, and which nanoparticles can be remotely activated.
2. The cell type of claim 1 , wherein the temperature sensitive channel is selected from the group consisting of TRPV1, TRPV2, TRPV3, TRPM8, chimeric TRP channels, and tandem pore domain potassium channels.
3. The cell type of claim 1 , wherein said cell is a stem cell.
4. The cell type of interest according to claim 1 , wherein the nanoparticles are activated when a radiofrequency field is applied thereto.
5. The cell type of interest according to claim 4 , wherein activated comprises increase in temperature.
6. The cell type of interest according to claim 1 , wherein the nanoparticles comprise ferritin, MagA, ceruloplasmin, or transferrin.
7. The cell type of interest according to claim 1 , wherein the nanoparticles are intracellularly expressed.
8. The cell type of interest according to claim 1 , wherein the nanoparticles comprise metallic or metal oxide nanoparticles.
9. The cell type of interest according to claim 1 , wherein the nanoparticles comprise a targeting moiety.
10. The cell type of interest according to claim 9 , wherein the targeting moiety comprises: antibody, streptavidin, or peptide.
11. The cell type of interest according to claim 1 , wherein the receptor is a G-protein coupled receptor.
12. A cell type of interest comprising ferritin nanoparticles that are selective for a temperature sensitive channel or receptor, and which ferritin nanoparticles can be remotely activated.
13. The cell type of interest according to claim 12 , wherein the ferritin nanoparticles comprise a targeting moiety.
14. The cell type of interest according to claim 12 , wherein the targeting moiety comprises: antibody, streptavidin, or peptide.
15. The cell type of interest according to claim 12 , wherein the receptor is a G-protein coupled receptor.
16. The cell type of interest according to claim 12 , wherein the cell is a stem cell.