Method and device for preparing a sample of biological material containing target cells and accompanying cells for extracting nucleic acids of the target cells
A method for preparing a sample of biological materials containing target cells and accompanying cells for extracting nucleic acids of the target cells includes accumulating the target cells of the sample by separating the target cells or the accompanying cells from the sample. The method also includes digesting the target cells via chemical and/or physical lysis in order to produce a target cell lysate containing the nucleic acids of the target cells. The method furthermore includes purifying the nucleic acids from the target cell lysate in order to extract the nucleic acids of the target cells.
1. A method for processing a sample of biological material containing target cells and accompanying cells for extracting nucleic acids of the target cells, the method comprising:
accumulating the target cells of the sample by removing the target cells or the accompanying cells from the sample;
disrupting the target cells with ultrasonic energy, wherein an ultrasonic generator is coupled to the filter in order to generate a target-cell lysate containing the nucleic acids of the target cells; and
purifying the nucleic acids from the target-cell lysate in order to extract the nucleic acids of the target cells, wherein:
disrupting the target cells includes disrupting the target cells on a filter, and
when the target cells are ultrasonically disrupted on the filter, the filter is completely or partially broken up into the filter's constituents.
2. The method as claimed in claim 1 , wherein:
accumulating the target cells includes removing the target cells from the sample by an accumulation filter.
3. The method as claimed in claim 2 , wherein:
accumulating the target cells includes cleaning the accumulation filter after removing the target cells from the sample.
4. The method as claimed in claim 1 , wherein:
accumulating the target cells includes adjusting the temperature of the sample to a lysis temperature for disrupting and predamaging the accompanying cells, and
accumulating the target cells includes lysing the accompanying cells by chemical lysis and enzymatically digesting the nucleic acids released from the accompanying cells.
5. The method as claimed in claim 4 , wherein:
the accompanying cells are lysed and the nucleic acids released from the accompanying cells are digested before, during, or after the temperature of the sample is adjusted to a lysis temperate, and
a viscosity of the sample is lowered when the accompanying cells are lysed and the nucleic acids released from the accompanying cells are digested.
6. The method as claimed in claim 1 , further comprising:
predisrupting the accompanying cells by a lysis buffer and/or coupling-in of ultrasound before accumulating the target cells of the sample.
7. The method as claimed in claim 1 , wherein accumulating the target cells includes diluting the sample.